Microwave-assisted acid hydrolysis of toluene diamine conjugates in urine samples for biomarkers in toluene diisocyanate analysis

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Title:	Microwave-assisted acid hydrolysis of toluene diamine conjugates in urine samples for biomarkers in toluene diisocyanate analysis
Authors:	Hui-Jung Yeh Wei-Chao Lin Shiann-Cherng Sheu Tung-Sheng Shih Lien-Huei Chen Ho-Yuan Chang
Contributors:	嬰幼兒保育系
Keywords:	Toluene diisocyanate Toluene diamine Microwave-assisted heating Hydrolysis
Date:	2007-06
Issue Date:	2010-01-11 14:08:07 (UTC+8)
Abstract:	Toluene diamines (TDAs) in urine have been used widely to determine the amount of toluene diisocyanate (TDI) absorbed by humans. Conventional hydrolysis to prepare a sample of urine takes approximately 16 h. An attempt is made to apply microwave-assisted heating (MAH) to reduce the duration of analysis. Urine collected from rats exposed to a mixture of 2,4- and 2,6-TDI was diluted with non-exposed human urine 1/1250-, 1/500- and 1/250-fold. The urine samples were hydrolyzed by both conventional heating and MAH. The hydrolysis efficiency obtained using MAH significantly exceeded that obtained using conventional heating. Hydrolysis by MAH required only 20 min, 48 times faster than with conventional heating. The use of the MAH method in hydrolysis was demonstrated to be reproducible, timesaving and efficient technique in measuring the concentration of urinary TDAs.
Relation:	Talanta 72(6) ： p.1527-1532
Appears in Collections:	[Dept. of Childhood Education and Nursery] Periodical Articles

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