Interactions between U-937 human macrophages and tyloxapol

doi:10.1016/j.colsurfb.2008.01.024

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/21732

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/21732

Title:	Interactions between U-937 human macrophages and tyloxapol
Authors:	Jung-hua Steven Kuo Yi-Lin Lin Jo-wen Tseng
Contributors:	藥物科技研究所
Keywords:	Tyloxapol Macrophage Reactive oxygen species Mitochondrial membrane potential Cell cycle
Date:	2008-07
Issue Date:	2009-10-13 09:29:38 (UTC+8)
Publisher:	Elsevier
Abstract:	Tyloxapol is reported to prevent macrophages from reacting to endotoxin. However, the intracellular responses that tyloxapol induces in macrophages are still not fully explored. Hence, the objective of this study was to evaluate the intracellular events in macrophages treated with tyloxapol and assess the antioxidant properties of tyloxapol in endotoxin-activated macrophages. Using flow cytometry, we examined intracellular responses in macrophages: reactive oxygen species (ROS) content, mitochondria membrane potential, and cell cycle profiles. We also assessed the antioxidant properties of tyloxapol in endotoxin-activated macrophages. Kinetic hydrogen peroxide production tended to decline with increasing doses. Tyloxapol produced a progressive increase followed by a decline in superoxide anion production in macrophages with increasing doses. Tyloxapol also caused unstable fluctuations in mitochondrial membrane potential. Apoptosis had developed at higher doses after 4 h of incubation time. After 2 h of tyloxapol-pretreatment, tyloxapol acted as an antioxidant only at lower doses. Most tyloxapol-pretreated cells at lower doses fully recovered from the changes in superoxide anion and hydrogen peroxide production. Our findings contribute to a better understanding of the molecular action of tyloxapol in macrophages and how it protects macrophages against endotoxin.
Relation:	Colloids and Surfaces B: Biointerfaces 64(2)：p.208-215
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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