Norsolorinic acid inhibits proliferation of T24 human bladder cancer cells by arresting the cell cycle at the G0/G1 phase and inducing a fas/membrane-bound Fas ligand-mediated apoptotic pathway

doi:10.1111/j.1440-1681.2008.05007.x

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 藥學系(所) > 期刊論文 > Item 310902800/21726

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標題:	Norsolorinic acid inhibits proliferation of T24 human bladder cancer cells by arresting the cell cycle at the G0/G1 phase and inducing a fas/membrane-bound Fas ligand-mediated apoptotic pathway
作者:	Clay CC Wang Yi-Ming Chiang Po-Lin Kuo Jiunn-Kae Chang Ya-Ling Hsu
貢獻者:	藥物科技研究所
關鍵字:	apoptosis bladder cancer cell cycle Fas norsolorinic acid
日期:	2008-11
上傳時間:	2009-10-13 09:29:29 (UTC+8)
出版者:	Wiley
摘要:	1. Norsolorinic acid, isolated from Aspergillus nidulans, has been shown to have antiproliferative activity in T24 human bladder cancer cells by arresting the cell cycle at the G(0)/G(1) phase and inducing apoptosis. The aim of the present study was to investigate the antiproliferative activity of norsolorinic acid in T24 human bladder cancer cells. 2. The effects of norsolorinic acid (1, 5, 10 and 20 mu mol/L) on the proliferation of T24 cells and on the distribution of cells within different phases of the cell cycle were investigated indirectly using an XTT assay and a flow cytometer, respectively. Factors affecting the cell cycle and apoptosis, including p53, p21, Fas receptor, Fas ligand (FasL) and caspase 8 activity, were examined using ELISA. 3. The results showed that norsolorinic acid inhibited proliferation of T24 cells in a dose-dependent manner, with an IC(50) of 10.5 mu mol/L. The effect involved the induction of cell cycle arrest at the G(0)/G(1) phase and apoptosis. 4. These results demonstrate that G(0)/G(1) phase arrest is due to increased expression of p21 in cells treated with norsolorinic acid (10 and 20 mu mol/L) for 24 h. Moreover, enhanced Fas and membrane-bound Fas ligand (mFasL) may be responsible for the apoptotic effect of norsolorinic acid. Thus, the present study reports, for the first time, that induction of p21 and the Fas/mFas ligand apoptotic system may participate in the antiproliferative action of norsolorinic acid in T24 human bladder cancer cells.
關聯:	Clinical and Experimental Pharmacology and Physiology 35(11)：p.1301 - 1308
显示于类别:	[藥學系(所)] 期刊論文

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