海巴戟天粗萃物清除1,1-Diphenyl-2-picryhyrazyl自由基能力與抑制EB病毒溶裂循環的效應

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Title:	海巴戟天粗萃物清除1,1-Diphenyl-2-picryhyrazyl自由基能力與抑制EB病毒溶裂循環的效應 1,1-Diphenyl-2-picryhyrazyl Radical Scavenging Activity and Inhibitory Effects of Epstein-Barr Virus Lytic Cycle by Crude Extracts of Morinda citrifolia L
Authors:	林翠品陳師瑩鄭筑勻葉東柏 Tsuey-Pin Lin Shih-Ying Chen Chu-Yun Cheng Dong-Bor Yeh
Contributors:	保健營養系(所)
Keywords:	Epstein-Barr病毒抗病毒海巴戟天 Epstein-barr virus antiviral Morinda citrifolia
Date:	2008-06
Issue Date:	2009-07-23 14:32:24 (UTC+8)
Abstract:	海巴戟天俗名諾麗(non)，在夏威夷與大平洋島嶼被廣泛利用，它有抗腫瘤與抗氧化等活性。本研究之目的將探討海巴戟天各部位水及乙醇粗萃物是否能夠抑制EB病毒溶裂循環，而且也將探討抑制EB病毒溶裂循環的效應與總酣類含量及1,1-Diphenyl-2-Picryrazyl (DPPH)自由基的清除能力是否具有關聯性。結果發現葉乙醇粗萃物比其他部位水及乙醇粗萃物含有較高的總多酚類含量及1,1-Diphenyl-2-Picryrazyl (DPPH)自由基的清除能力。同時也證明這個組萃物在200µg/mL時對EB病毒溶裂循環時期的蛋白質表現具有明顯抑制作用，而且發現葉乙醇粗萃物中多酚類物質可能具有抗氧化及抗病毒的效應。此外，也發現葉乙醇粗萃物明顯抑制作用的濃度對P3HR1細胞不具有毒性，所以具有開發成為抗EB病毒製劑的價值。 Morinda citrifolia L. (Rubiaceae), commonly known as noni, is used in the Hawaiian and Tahitian islands, and has antitumor and antioxidant activities. The purpose of this study was to investigate whether the crude extracts of M. citrifolia inhibit the lytic cycle of Epstein-Barr virus and the possible relation among antiviral activities, total phenolic contents, and 1,1-diphenyl-2-picryhyrazyl (DPPH) radical-scavenging capacity. We found that the ethanolic extract from leaves of M. citrifolia had the highest Total phenolic contents and DPPH radical-scavenging activity of aqueous and ethanolic extracts. Meanwhile, results demonstrated that 200 �g/mL of this ethanolic extract effectively inhibited the expression of Epstein-Barr virus lytic proteins during the viral lytic cycle, thus showing that the phenolic extract may have antioxidant and antiviral effects. Furthermore, this extract was not toxic toward P3HR1 cells. Thus, it can potentially be developed as an anti-EBV drug.
Relation:	臺灣營養學會雜誌 33(2)：p.77-85
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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