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    標題: Protective effects of pu-erh tea on LDL oxidation and nitric oxide generation in macrophage cells
    作者: Bor-Sen Wanga
    Hui Mei Yub
    Lee-Wen Changa
    Wen-Jye Yena
    Pin-Der Duh
    王柏森
    晏文潔
    張瓈文
    杜平悳
    貢獻者: 食品科技系
    關鍵字: Pu-erh tea
    LDL oxidation
    Reactive oxygen species
    Nitric oxide
    iNOS expression
    Gallic acid
    Epicatechin
    Caffeine
    日期: 2008-07
    上傳時間: 2009-07-23 14:32:22 (UTC+8)
    摘要: Effects of water extract of pu-erh tea (WEPT) on low density of lipoprotein (LDL) oxidation and their regulation of nitric oxide in macrophage RAW 264.7 cells were determined. The results showed that WEPT significantly scavenged H2O2 in a concentration-dependent manner. In the range of 0–0.1 mg/mL, the inhibitory action on LDL oxidation increased with increasing concentration of WEPT. In addition, WEPT scavenged O2− and inhibited the activity of xanthine oxidase, suggested that WEPT showed effectively inhibitory effect on oxidative stress. WEPT at 200 μg/mL not only increased the glutathione (GSH) content by 41.5-fold but also enhanced activity of glutathione peroxidase (GPX), glutathione reductase (GR), glutathione transferase (GST) and catalase (CAT) in 3T3 cells by 1.8-, 2.6-, 10.9- and 4.3-fold compared to the control, respectively, revealing that WEPT may positively modulate the GSH and antioxidant enzyme system. Moreover, WEPT scavenged NO generated by sodium nitroprusside (SNP). The expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-activated macrophage RAW 264.7 cells was inhibited by WEPT. Reverse transcription-polymerase chain reaction (RT-PCR) analysis indicated that the iNOS mRNA expression was suppressed by WEPT. Furthermore, the down-regulated degradation of IκB-α by WEPT was found, indicating that WEPT suppressed iNOS enzyme expression as a result of preventing nuclear factor-kappa B (NF-κB) activation. The HPLC analyses showed that gallic acid, epicatechin and caffeine, which are polyphenolics with bioactive action, were found in WEPT. Overall, these findings suggest that pu-erh tea may play a crucial role in preventing such oxidation-related diseases as atherosclerosis and other types of vascular diseases.
    關聯: LWT - Food science and technolog 41(6):p.1122-1132
    顯示於類別:[ 食品科技系 ] 期刊論文

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