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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/21613


    標題: Zebrafish anti-apoptotic protein zfBcl-xL can block betanodavirus protein α-induced mitochondria-mediatedsecondary necrosis cell death
    作者: Horng-Cherng Wu
    Chuan-Sheng Chiu
    Jen-Leih Wu
    Hong-Yi Gong
    Ming-Chyuan Chen
    Ming-Wei Lu
    Jiann-Ruey Hong
    吳鴻程
    貢獻者: 食品科技系
    關鍵字: Nervous necrosis virus
    Protein α
    Mitochondria
    zfBcl-xL
    Apoptosis
    Secondary necrosis cell death
    日期: 2008-04
    上傳時間: 2009-07-23 14:32:19 (UTC+8)
    摘要: Betanodavirus protein α induces cell apoptosis or secondary necrosis by a poorly understood process. In the present ork, red spotted grouper nervous necrosis virus (RGNNV) RNA 2 was cloned and transfected into tissue culture cells (GF-1) which then underwent apoptosis or post-apoptotic necrosis. In the early apoptotic stage, progressive phosphatidylserine externalization was evident at 24 h post-transfection (p.t.) by Annexin V-FLUOS staining. TUNEL assay revealed poptotic cells at 24–72 h p.t, after which post-apoptotic necrotic cells were identified by acridine orange/ethidium romide dual dye staining from 48 to 72 h p.t. Protein α induced progressive loss of mitochondrial membrane potential (MMP) which was detected in RNA2-transfected GF-1 cells at 24, 48, and 72 h p.t., which correlated with cytochrome c release, especially at 72 h p.t. To assess the effect of zfBcl-xL on cell death, RNA2-transfected cells were co-transfected with zfBcl-xL. Co-transfection of GF-1 cells prevented loss of MMP at 24 h and 48 h p.t. and blocked initiator caspase-8 and effector caspase-3 activation at 48 h p.t. We conclude that RGNNV protein α induces apoptosis followed by econdary necrotic cell death through a mitochondria-mediated death pathway and activation of caspases-8 and -3.
    關聯: Fish & shellfish immunology 24(4):p.436-449
    Appears in Collections:[ 食品科技系 ] 期刊論文

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