Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells

doi:10.1016/j.bbrc.2007.12.135

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/21574

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標題:	Lysophosphatidic acid stimulates thrombomodulin lectin-like domain shedding in human endothelial cells
作者:	Hua-Lin Wu Chi-Iou Lin Yuan-Li Huang Pin-Shern Chen Cheng-Hsiang Kuo Mei-Shing Chen Georgiana Cho-Chen Wu Guey-Yueh Shi Hsi-Yuan Yang Hsinyu Le
貢獻者:	生物科技系
關鍵字:	Thrombomodulin Lysophosphatidic acid Endothelial cells
日期:	2008-02
上傳時間:	2009-07-21 14:07:03 (UTC+8)
出版者:	Elsevier
摘要:	Thrombomodulin (TM) is an anticoagulant glycoprotein highly expressed on endothelial cell surfaces. Increased levels of soluble TM in circulation have been widely accepted as an indicator of endothelial damage or dysfunction. Previous studies indicated that various proinflammatory factors stimulate TM shedding in various cell types such as smooth muscle cells and epithelial cells. Lysophosphatidic acid (LPA) is a bioactive lipid mediator present in biological fluids during endothelial damage or injury. In the present study, we first observed that LPA triggered TM shedding in human umbilical vein endothelial cells (HUVECs). By Cyflow analysis, we showed that the LPA-induced accessibility of antibodies to the endothelial growth factor (EGF)-like domain of TM is independent of matrix metalloproteinases (MMPs), while LPA-induced TM lectin-like domain shedding is MMP-dependent. Furthermore, a stable cell line expressing TM without its lectin-like domain exhibited a higher cell proliferation rate than a stable cell line expressing full-length TM. These results imply that LPA induces TM lectin-like domain shedding, which might contribute to the exposure of its EGF-like domain for EGF receptor (EGFR) binding, thereby stimulating subsequent cell proliferation. Based on our findings, we propose a novel mechanism for the exposure of TM EGF-like domain, which possibly mediates LPA-induced EGFR transactivation.
關聯:	Biochemical and biophysical research communications 367(1)：p.162-168
显示于类别:	[生物科技系(所)] 期刊論文

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