Arecoline and the 30-100 kDa fraction of areca nut extract differentially regulate mTOR and respectively induce apoptosis and autophagy: a pilot study

doi:10.1007/s11373-008-9273-8

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/21572

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/21572

Title:	Arecoline and the 30-100 kDa fraction of areca nut extract differentially regulate mTOR and respectively induce apoptosis and autophagy: a pilot study
Authors:	Shyun-Yeu Liu Mei-Huei Lin Yu-Rung Hsu Ya-Yun Shih Wei-Fan Chiang Chin-Hai Lee Ta-Hsiung Chou Young-Chau Liu
Contributors:	生物科技系
Keywords:	Arecoline Areca nut Apoptosis Autophagy Mammalian target of rapamycin (mTOR)
Date:	2008-11
Issue Date:	2009-07-21 14:06:59 (UTC+8)
Abstract:	Areca nut (AN) is recognized as a human carcinogen; however, few studies of the cytotoxic effects of AN ingredients on cells have been reported. In Taiwan, AN, lime and inflorescence of Piper betle are the common components of betel quid (BQ). We recently noticed that extract of AN (ANE), but not those of lime and inflorescence of Piper betle, induces rounding cell morphology and nuclear shrinkage in different types of carcinoma cells. In this study, the rounding cell activity was first traced to the partially purified ≥10 kDa fraction (ANE ≥ 10 K) and subsequently to the 30–100 kDa fraction (ANE 30–100 K). ANE and ANE ≥10 K stimulated nuclear shrinkage (P < 0.001 in both cases) and the clearance of the cytoplasm. ANE, ANE ≥ 10 K, and ANE 30–100 K induced the cleavage of LC3-I (P < 0.05, 0.01, and 0.05, respectively) and the emergence of autophagic vacuoles (AVs) and acidic vesicles. On the other hand, arecoline (Are, the major alkaloid of AN) triggered caspase-3 activation, peri-nuclear chromatin condensation, and micronucleation. Meanwhile, ANE 30–100 K, but not Are, inhibited the phosphorylation of the mammalian target of rapamycin (mTOR)-Ser2448. In conclusion, this study demonstrates that different AN ingredients exerting differential impact on mTOR-Ser2448 phosphorylation are capable of triggering apoptosis and autophagy.
Relation:	Journal of biomedical science 15(6)：p.823-831
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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