Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/21568
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    標題: Iridovirus Bcl-2 protein inhibits apoptosis in the early stage of viral infection
    作者: Pei-Wen Lin
    Yi-Jen Huang
    Joseph Abraham Christopher John
    Ya-Nan Chang
    Chung-Hsiang Yuan
    Wen-Ya Chen
    Chiao-Hwa Yeh
    San-Tai Shen
    Fu-Pang Lin
    Wen-Huei Tsui
    Chi-Yao Chang
    陳玟雅
    貢獻者: 生物科技系
    關鍵字: Apoptosis
    Bcl-2
    Grouper
    Iridovirus
    Mitochondria
    日期: 2008-01
    上傳時間: 2009-07-21 14:06:49 (UTC+8)
    摘要: The grouper iridovirus (GIV) belongs to the family Iridoviridae, whose genome contains an antiapoptotic B-cell ymphoma (Bcl)-2-like gene. This study was carried-out to understand whether GIV blocks apoptosis in its host. UV- rradiated grouper kidney (GK) cells underwent apoptosis. However, a DNA fragmentation assay of UV-exposed GK cells after GIV infection revealed an inhibition of apoptosis. The UV- or heat-inactivated GIV failed to inhibit apoptosis, mplying that a gene or protein of the viral particle might contribute to an apoptosis inhibitory function. The DNA ladder ssay for GIV-infected GK cells after UV irradiation confirmed that apoptosis inhibition was an early process which ccurred as early as 5 min post-infection. A GIV-Bcl sequence comparison showed distant sequence similarities to that of uman and four viruses; however, all possessed the putative Bcl-2 homology (BH) domains of BH1, BH2, BH3, and BH4, s well as a transmembrane domain. Northern blot hybridization showed that GIV-Bcl transcription began at 2 h post- nfection, and the mRNA level significantly increased in the presence of cycloheximide or aphidicolin, indicating that this GIV-Bcl is an immediate-early gene. This was consistent with the Western blot results, which also revealed that the virion carries the Bcl protein. We observed the localization of GIV-Bcl on the mitochondrial membrane and other defined ntracellular areas. By immunostaining, it was proven that GIV-Bcl-expressing cells effectively inhibited apoptosis. Taken together, these results demonstrate that GIV inhibits the promotion of apoptosis by GK cells, which is mediated by the mmediate early expressed viral Bcl gene.
    關聯: Apoptosis 13(1):p.165-176
    显示于类别:[生物科技系(所)] 期刊論文

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