海巴戟天之葉萃取物的抗氧化活性分析

CNU IR > Chna Nan Annual Bulletin > No.29 (2003) > Item 310902800/21379

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Title:	海巴戟天之葉萃取物的抗氧化活性分析 Evaluation of Antioxidative Activity in Noni Leaf Extracts
Authors:	陳師瑩陳靜慧王櫻娟江靜欣鍾玉玲葉東柏
Contributors:	保健營養學系食品衛生系生物科技系
Keywords:	海巴戟天二氧化碳超臨界萃取環磷醯胺抗氧化活性 Noni Supercritical carbon dioxide extraction Cyclophosphamide Antioxidative activity
Date:	2003
Issue Date:	2009-06-11 13:32:55 (UTC+8)
Abstract:	本實驗利用超臨界二氧化碳萃取技術(SFE-CO2)、99.5% EtOH、50% EtOH及去離子水萃取海巴戟天(台灣2號) 的葉(Noni-L)，並就萃取物進行體外抗氧化活性分析。抗氧化能力以Trolox當量的抗氧化能力、DPPH自由基清除能力、清除氫氧自由基(‧OH)能力以及螯合鐵的能力為指標。在考慮到抗氧化活性與成本效益下，各種萃取模式中是以去離子水萃取海巴戟天的葉最適當。將18隻成熟的雄性大白鼠隨機分為3組，並餵食4週。其中一組大白鼠於實驗最後兩週每隔一天進行腹腔注射生理食鹽水，當作是控制組(CK)；其餘兩組則腹腔注射環磷醯胺(25 mg/kg)，一組在飼料中添加50 mg/kg海巴戟天葉之去離子水萃取物，作為實驗組(Noni-LW)，另一組未添加任何測試物質之飼料，則作為對照組(CP)。實驗結果顯示Noni-L的水萃取物可以顯著保護大白鼠因環磷醯胺所誘發血球細胞之脂質過氧化現象，也有提昇肝臟及血漿的總抗氧化活性，但未達顯著差異。 Taiwan number 2 Noni leaf (Noni-L) were extracted by a supercritical carbon dioxide extraction (SFE-CO2) technique or using 99.5% EtOH, 50% EtOH or deionized water as extraction solvent. Trolox equivalent antioxidant capacity, the scavenging of Ñ、Ñ-diphenyl-Ò-picrylhydrazyl (DPPH) radical, the scavenging of hydroxyl radical, and the binding capacity of iron were used to indicat the antioxidant activities of Noni-L. We considered both the antioxidant activities and cost-benefit, the ideal condition of our various operation to extract the antioxidant from Noli-L was deionized water as extraction solvent. Eighteen adult male rats were divided into three groups randomly and fed with rodent diet for 4 weeks. One group were injected i.p. for the last 2 week with normal saline on every other day and served as the control group (CK). Another group were injected i.p. cyclopho-sphamide (25 mg/kg) and fed with rodent diet combined with 50 mg/kg deionized water extract powder from noni leaf on every day and served as the experimental group (Noni-LW). The other group as a comparison group (CP) treated with the same as Noni-LW group, but fed with rodent diet only in the experiment. In conclusions, administration of Noni-L extracted by deionized water to cyclophosphamide-injected rats howed the protective properties of the peroxidation of lipid in rats’ blood cells. Furthermore , it may be helpful but not significant in elevating total antioxidative activity of rats’ liver and plasma.
Relation:	嘉南學報(科技類)29期：p.87-96
Appears in Collections:	[Chna Nan Annual Bulletin] No.29 (2003) [Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Food Science & Technology] Periodical Articles [Dept. of Biotechnology (including master's program)] Periodical Articles

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