Cloning and Sequence Analysis of fenY， A Gene Involved in Fengycin Synthesis

CNU IR > Chna Nan Annual Bulletin > No.29 (2003) > Item 310902800/21368

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Title:	Cloning and Sequence Analysis of fenY， A Gene Involved in Fengycin Synthesis 豐原素合成基因，fenY的選殖與序列分析
Authors:	林翠品
Contributors:	保健營養系
Keywords:	Fengycin Bacillus subtilis Nonribosomal peptide synthesis 豐原素枯草桿菌非核糖體
Date:	2003
Issue Date:	2009-06-11 10:12:43 (UTC+8)
Abstract:	A Bacillus subtilis F29-3 mutant defective in fengycin biosynthesis was isolated by Tn917 mutagenesis. Southern hybridization revealed that Tn917 in this mutant is inserted at a location outside of the chromosomal region containing the fengycin synthetase operon. Sequencing analysis showed that the transposon is inserted in an unknown gene fenY in the nasA operon. The protein encoded by this gene potentially contains three phosphorylation sites and may be involved in the regulation fengycin synthesis. 枯草桿菌F29-3突變株的篩選是以Tn917轉位子進行突變作用所得到的，將這些突變株以南方墨點法分析，發現FE8突變株中Tn917轉位子所突變的位子不在豐原素peptide synthetase操縱組上。進一步以Tn917轉位子所突變的附近染色體序列作為探針篩選枯草桿菌F29-3基因庫，再分析DNA序列發現FE8突變株中Tn917轉位子所突變的位子是在與氮代謝有關的nasA操縱組附近的基因(fenY)，FenY蛋白質序列分析發現含有三個磷酸化的位子，所以推測FenY可能參與在豐原素合成的調控上。
Relation:	嘉南學報(科技類)29期：p.128-135
Appears in Collections:	[Chna Nan Annual Bulletin] No.29 (2003) [Dept. of Health and Nutrition (including master's program)] Periodical Articles

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