Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/21368
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17776/20117 (88%)
Visitors : 10938687      Online Users : 150
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    CNU IR > Chna Nan Annual Bulletin > No.29 (2003) >  Item 310902800/21368
    Please use this identifier to cite or link to this item:

    Title: Cloning and Sequence Analysis of fenY, A Gene Involved in Fengycin Synthesis
    Authors: 林翠品
    Contributors: 保健營養系
    Keywords: Fengycin
    Bacillus subtilis
    Nonribosomal peptide synthesis
    Date: 2003
    Issue Date: 2009-06-11 10:12:43 (UTC+8)
    Abstract: A Bacillus subtilis F29-3 mutant defective in fengycin biosynthesis was isolated by Tn917 mutagenesis. Southern hybridization revealed that Tn917 in this mutant is inserted at a location outside of the chromosomal region containing the fengycin synthetase operon. Sequencing analysis showed that the transposon is inserted in an unknown gene fenY in the
    nasA operon. The protein encoded by this gene potentially contains three phosphorylation sites and may be involved in the regulation fengycin synthesis.
    枯草桿菌F29-3突變株的篩選是以Tn917轉位子進行突變作用所得到的,將這些突變株以南方墨點法分析,發現FE8突變株中Tn917轉位子所突變的位子不在豐原素peptide synthetase操縱組上。進一步以Tn917轉位子所突變的附近染色體序列作為探針篩選枯草桿菌F29-3基因庫,再分析DNA序列發現FE8突變株中Tn917轉位子所突變的位子是在與氮代謝有關的nasA操縱組附近的基因(fenY),FenY蛋白質序列分析發現含有三個磷酸化的位子,所以推測FenY可能參與在豐原素合成的調控上。
    Relation: 嘉南學報(科技類)29期:p.128-135
    Appears in Collections:[Chna Nan Annual Bulletin] No.29 (2003)
    [Dept. of Health and Nutrition (including master's program)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    v29_128_135.pdf61KbAdobe PDF556View/Open

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback