遊憩用水包含相當廣泛，本研究針對溫泉、水療池的水質維持進行探討。傳統的消毒方式就是加氯消毒，但氯系消毒藥劑有儲存運送與操作之高風險。本研究即以新穎電解技術產生電解消毒液(MIOX)應用於遊憩用水消毒作為研究主軸，探討電解消毒技術對溫泉及水療池中水質之影響，透過水質檢驗評估與成本分析，以評估最適操作條件。
本研究利用電解消毒液(MIOX)，應用於水療池與溫泉池進行消毒研究。首先於某一SPA 水療池進行研究，原水pH 為7.9~8.3，溫度約為29℃，氧化還原電位約為+l 00 mV，導電度約為350μS / cm，結果顯示，需將水中自由餘氯濃度控制在0.6 mg / L as Cl2，方可將總菌落數抑制在符合法規標準；接下來於某一溫泉飯店進行溫泉池消毒評估，此一溫泉屬鹼性碳酸氫鹽泉，原泉pH值約為8.9，溫度約為38℃，氧化還原電位約為-26 mV , 導電度約為3900μS / cm，結果顯示，溫泉水在pH 值小於8.9時，需將水中自由餘氯控制在往0.44mg/L as Cl2以上；而pH值高於8.9時，則需將水中自由餘氯控制在0.55mg/ L as Cl2以上，方可將總菌落數抑制在符合法規標準。在加藥成本計算，若以符合管制微生物之考量前提，針對本研究利用電解消毒液(MIOX)消毒處理每噸水，水療池及溫泉池的加藥成本分別需0.04元(新台幣，NT)/每噸水及0.02元(新台幣，NT)/每噸水。 The aim of this study was to synthosize flunitrazepam (FM2)-protein bioconjugates and produce polylonal antibodies (PAbs) against FM2 (anti-FM2 PAbs). ICR mice were intraperitoneally immunized with FM2-protein bioconjugates every two weeks. Because the rnolecular weight of FM2 is very small, it is difficult to induce antibodies production for mice. The FM2 molecules were conjugated with bovine serum albumin (BSA) and sericin (Ser), respectively Hyperimmune ICR mice produced anti-FM2 PAbs after injected with 0.5 mL pristane, and injected with NS-1 myeloma cells two weeks later. Ascites and serum were collected from ICR mice, but before the mice had difficulty moving, and anti-FM2 PAbs was purified by using HitrapTM rProtein A column. In this study, the drug-proteins bioconjugates had been successfully produced. FM2-BSA bioconjugate was as the immunogen for mice immunization and FM2-Ser bioconjugate was as the antigen for Enzyme-link Immunosorbent Assay ( ELISA ). Finally, the production and primary purification of anti-FM2 PAbs were performed. The concentration of PAbs obtained was 9.49mg/mL. Then , the anti-FM2 PAbs were stored by freeze-dry method.
