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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/21202

    標題: Specific Primers for Rapid and Sensitive Identification of Vibrio vulnificus
    作者: Yu-Chung Chen
    Jer-Horng Su
    Wen-Teish Chang
    貢獻者: 生物科技系
    關鍵字: Bacterial identification
    Blue fluorescent protein
    Vibrio vulnificus
    日期: 2005
    上傳時間: 2009-04-27 13:15:01 (UTC+8)
    摘要: A primer pair designed from vvbfp (Vibrio vulnificus blue fluorescent protein) of V. vulnificus amplified a 251 bp PCR fragment from all clinical isolates of V. vulnificus and failed to amplify any fragment from other clinical pathogens. The sensitivity of the primer pair is as little as 1 fg of chromosomal DNA and 60 cells of V. vulnificus. In a scientific evaluation of specimens, a total of 22 samples including serum specimens and wound aspirates from septicemic mice were screenedy PCR. Thirteen (92.9%) of the 14 V.vulnificus culture-positive specimens gave positive results. Three (37.5%) of the culturenegative specimens yielded positive results. The primer set gives high sensitivity and specificity in a one-step PCR protocol, an important criterion for unambiguous bacterial identification.
    關聯: 嘉南學報(科技類)31期:p.19-27
    Appears in Collections:[嘉南學報] 31 期 (2005)
    [生物科技系(所)] 期刊論文

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