|摘要: ||本研究乃探討石蓴過氧化物水解酶(HPLS)之特性。石蓴粗抽出液經MacroPrep-Q離子交換及Sephacryl S-300膠過濾後,過氧化物水解酶純化了31.7倍。石蓴HPLS的最適pH及溫度分別為pH6.6及34.degree.C,於pH 6-8、溫度37.degree.C以下, HPLS活性安定。對於不同脂肪酸過氧化物之反應性以18: 2-13OOH及18:3-13OOH最高,對18:2-9OOH及18:3-9OOH不具反應性。探討八種不同過氧化物水解酶抑制劑之影響,以Quercetin之抑制效果最高,其次為HgCl/sub 2/,BHA, PG,Esculetin,NDGA,BHT及Esculin。|
Hydroperoxide lyase (HPLS) was isolated and purified 32-fold from sea algae (Ulva lactuca) using MacroPrep-Q ion exchange and gel filtration on Sephacryl S-300. The optimal pH of the algal HPLS was 6.6, and optimal temperature was 34.degree.C. At pH ranged 6 to 8 and temperature below 37.degree.C, the algal HPLS was stable. The algal HPLS showed the highest reactivity toward 18:2-13OOH and 18: 3-13OOH, while no activity was observed on 18:2-9OOH and 18:3-9OOH. Eight synthetic HPLS inhibitors including BHT, BHA, quercetin, esculetin, esculin, NDGA, PG, and HgCl/sub 2/ were used in this study. Among them, quercetin showed the highest inhibition, followed by HgCl/sub 2/, BHA, PG, esculetin, NDGA, BHT and esculin.