The effectiveness of glutathione as an anticataract agent is limited by poor drug delivery and limited ocular bioavailability. In this study, liposomes containing glutathione have been prepared from different phospholipids using a modification of the ethanol injection technique. The in vitro transcorneal delivery of liposomal glutathione through pig's eyes were studied. Then, the interaction occurring between liposome and pig's cornea was determined by DSC. In the in vitro perfusion studies, the results show that the transcorneal flux of the GSH-loaded liposomes was significantly (p<0.05) higher than that of the free drug solution. For the three lipids investigated drug corneal penetration decreased in the order DSPC>DPPC>DMPC. Moreover, the loading of the drug in the cornea with liposome was higher, and that drainage of the liposomal GSH from the cornea was slower than for the solution form. Liposome-encapsulation is able to prolong the residence time of drug in eye and that results in sustained therapeutic effect. To transfer the drug through the cornea did not cause significantly alternation in structure of the tissue and to maintain the pharmacological activity, liposome-encapsulation of the drug is a good choice.