Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/167
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/167


    Title: 建立針對含雙硫鍵蛇毒蛋白的大腸菌表達系統
    Authors: 江建民
    Contributors: 生物科技系
    Date: 2007
    Issue Date: 2008-05-13 14:28:49 (UTC+8)
    Publisher: 台南縣：嘉南藥理科技大學生物科技系
    Abstract: 在本計畫中，我們成功利用大腸菌來表達台灣眼鏡蛇心臟毒CTX A3。我們利用融合蛋白ZZ來增加水溶性，利用細胞間質表達的方式來增加雙硫鍵的正確形成。台灣眼鏡蛇心臟毒素主要被表達在細胞外，胞內細胞間質、細胞質內及胞涵體內都不多。利用親合性管柱，我們可以純化到大腸菌所表達的重組融合心臟毒素A3，這可以提供未來針對心臟毒素進行突變以改良其性質。
    Relation: 計畫編號 : CN9620
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Chna Project

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