Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/1127
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    Title: 有益微生物乳酸菌之DNA序列分析
    Authors: 陳椒華
    Contributors: 食品科技系
    Date: 2003
    Issue Date: 2008-06-26 15:06:55 (UTC+8)
    Publisher: 台南縣:嘉南藥理科技大學食品科技系
    Abstract: 本研究從市售鮮奶中分離出八株似乳酸菌菌株,經凝乳試驗、革蘭氏染色法、型態觀察及catalase試驗等測試,初步判定其為類乳酸菌菌株。經再進一步利用乳酸菌快速鑑定套組(API 50CHL)進行菌種鑑定,分別得到與Pedio.pentosaceus、Lacto.brevis及Lacto.para.paracasei等菌種有高相似度的菌種。為進一步確認所分離乳酸菌之種源性,本實驗再進行16s rRNA序列分析。經從DNA資料庫分析,選定數株乳酸菌之16s rRNA gene保守性序列當primers,再進行本研究菌株之PCR增幅作用,所得PCR增幅產物再進行DNA序列分析。本研究所分離之類乳酸菌菌株的16s rRNA gene序列分析經與GeneBank(GCG)裡之乳酸菌16s rRNA gene序列進行比對,得到一株與Lacto.para.paracasei之16s rRNA gene序列相似度極高之菌株,其分析結果與API 50CHL之分析結果一致。
    Relation: 計畫編號:CNFH9205
    Appears in Collections:[Dept. of Food Science & Technology] Chna Project

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