本研究旨在分析與腫瘤相關的第一型基質金屬蛋白酵素﹝matrix
metalloproteinase-1，MMP-1﹞，在南台灣的口腔腫瘤中所扮演的角色。我們利用半定量性的反轉錄DNA 聚合酵素連鎖反應﹝reverse transcription-polymerasechain reaction﹞以及第一型基質金屬蛋白酵素與甘油醛-3-磷酸去氫酵素﹝glyceraldehyde 3-phosphate dehydrogenase﹞基因的啟動子﹝primers﹞，在39個口腔腫瘤樣本與腫瘤旁的組織中偵測MMP-1 的表現情形。結果顯示在所有39對組織中，與腫瘤周圍的組織相較之下，MMP-1 的轉錄本均偏好表現於腫瘤塊中。此外，其中有5 對組織當初在冰存前已切成數塊樣本，因而可進一步製作成組織萃取液並以西方轉印法﹝Western blot﹞偵測MMP-1 蛋白。結果在這5 對組織的腫瘤樣本中，MMP-1 蛋白均有表現且表現量亦較周圍組織更高。我們因此結論在南台灣的口腔腫瘤中MMP-1 可能普遍均有表現且集中於腫瘤塊中，此外，MMP-1 也可能在整個口腔腫瘤癌化的發展過程中扮演了重要的角色。 The objective of this study is to analyze roles of tumor-associated matrix
metalloproteinase-1 (MMP-1) in oral tumor patients from southern Taiwan.
Thirty-nine surgical specimens of oral tumors (Ts) and their neighboring tissues (Ns)
were subjected to the semi-quantitative reverse transcription-polymerase chain
reaction (RT-PCR) with the primers of MMP-1 and glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) genes. All of the 39 specimen pairs showed preferential
expression patterns of MMP-1 messages in Ts than in Ns (100%). Furthermore, 5
pairs of them, originally stored with more than two pieces of Ts and Ns, were
available for further homogenate preparation and MMP-1 protein detection by
Western blot. The results demonstrated that MMP-1 proteins were also predominantly
expressed in Ts than in Ns in all the 5 specimens tested. We conclude that MMP-1
may be universally and preferentially expressed within tumor masses and plays
important roles throughout the entire developmental process of oral cancers in
southern Taiwan.
