半枝蓮抗癌活性成分之抽取及分離研究

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Title:	半枝蓮抗癌活性成分之抽取及分離研究 The extraction and separation of the anticancer constituents from Scutellaria barbata don
Authors:	廖恭顯 Kung-Hsien Liao
Contributors:	魏明治嘉南藥理科技大學：生物科技研究所
Keywords:	中草藥活性成份研究 Scutellaria barbata don extraction
Date:	2009
Issue Date:	2009-03-12 11:33:09 (UTC+8)
Abstract:	半枝蓮是我國民間生藥材之一，具有抗癌作用，常用於肝臟疾病之治療且經現代藥理實驗發現，半枝蓮之抗癌活性wogonin、luteolin、apigenin、naringenin、baicalein、baicalin 及 protocatechuic acid等具有抗癌之作用。本研究以溶劑與超臨界二氧化碳，分別抽取半枝蓮中上述等保肝與抗腫瘤成份。探討各種抽取條件，如不同溶劑（水、乙醇、丙酮及正己烷等）、抽取的溫度與時間，以及超臨界二氧化碳抽取的溫度與壓力等變因對於各成份抽取量之影響。各種抽取條件之抽取液經由高效率液相層析儀(HPLC)定量分析，以作為最佳抽取條件之指標。在溶劑抽取方面，顯示以40-80%的乙醇與50%丙酮水溶液作為抽取劑，在50℃下抽取1小時，naringenin等六種黃酮類成份具有較佳的抽取產率。對於protocatechuic acid而言，其抽取產率於純水中較高。於壓力117-154 bar與溫度40-50℃下，以超臨界二氧化碳抽取半枝蓮時，其抽取效果均不佳。關鍵詞：半枝蓮、超臨界二氧化碳抽取、溶劑抽取 The tradition chinese medicine“Scutellaria barbata don”has the anticancer and hepatoprotective constituents. Results obtained from the pharmacological experiments demonstrated that some constituents, such as wogonin、luteolin、apigenin、naringenin、baicalein、baicalin and protocatechuic acid in Scutellaria barbata don have shown significant inhibitory activity on the cancer. This study extracted the hepatoprotective and antitumor activities, such as wogonin、luteolin、apigenin、naringenin、baicalein、baicalin and protocatechuic acid from Scutellaria barbata bon using solvent and supercritical carbon dioxide extraction. With pure water, different percentage of alcohol aqueous solution, acetone and n-hexane as extractants, the effects of extraction factors, temperature and extraction time on extraction yield were studied. On the other hand, the effects of temperature and pressure on extraction yield were also discussed using supercritical fluid extraction. Analyses of the extracts were performed by high-performance liquid chromatography to determine the contents of components. For all the analytes of the experimental conditions providing the highest extraction yield inside the experimental domain considered were found. Solvent extraction results showed that the optimum conditions for six flavonids constituents, such as naringenin etc. were achieved by using 40-80% ethanol and 50% acetone at 50℃ for extraction 1 h. The content of protocatechuic acid in ethanol and acetone extracts was low, but water extracts showed higher content. On the other hand, using carbon dioxide alone in supercritical fluid extraction at pressures from117 to 154 bar and temperatures from 40 to 50℃, the extraction yield of Scutellaria Barbata Don was not satisfactory. Key words: Scutellaria barbata don, Supercritical carbon dioxide extraction, Solvent extraction, wogonin,baicalein, baicalin
Relation:	校內校外均不公開，學年度：97，74頁
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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