本研究目的是探討海巴戟天葉乙醇萃取物之上清液 (LES) 對餵食高脂飲食小鼠之氧化狀態的影響。將 70 隻小鼠隨機分為下列 7 組，AIN 76A飲食組（A）、高脂飲食組（F） 和 F 組分別添加 0.025 ％ quercetin（Q）、0.005 ％ Rutin （RL）、0.025 ％ Rutin （RH）、0.2 ％ LES （LEL）以及 1 ％ LES（LEH），飼養為期 80 天。結果顯示攝食 Quercetin、Rutin 和 LES之實驗各組與 F 組相比較，皆不影響小鼠的最終體重、每日食物攝食量、攝食效率和相對肝臟重量。然而在血液分析中，LEH 組可顯著增加小鼠體內血球總抗氧化能力（TEAC）及降低脂質過氧化作用，並可提升葡萄糖六磷酸脫氫酶（G6PD）的活性。此外，LEH 組有較高的紅血球細胞數目（RBC）、血紅素與血球細胞容積，此結果顯示 LEH 組可保護紅血球對抗高脂飲食所誘發的氧化壓力。在肝臟中，LEH 組也可能增加超氧歧化酶（SOD）與 G6PD 的酵素活性。綜合 RL及 RH 組在小鼠血球細胞及肝臟中的抗氧化能力發現，與 LEL及 LEH 組有相同的趨勢。研究結論顯示 LES 對於高脂飲食所造成血球細胞與肝臟組織的氧化壓力有保護作用，而 LES 對此保護作用的成效與使用之劑量有關，並且保護氧化壓力的效果可能不僅是 Rutin 所發揮的效果，可能也有其他的生物活性成分。 The aim of this study was to evaluate the influence of supernatant from ethanolic extracts of Morinda citrifolia L. leaf ( LES ) on the oxidative status of mice fed with high fat diet. Seventy mice were divided into seven groups. The AIN 76A regular diet (A) or high-fat diet (F), and F group with either 0.025% quercetin (Q), 0.005% and 0.025% rutin (RL, RH), 0.2% and 1%LES (LEL, LEH) for a 80-day period. Results indicated that quercetin, rutin and LES do not affect final body weight, daily food intake, feed efficiency and relative liver weight of mice when compared to F group. However, LEH group could significantly increase trolox equivalent antioxidant capacity (TEAC), decrease lipid peroxidation and mild increase glucose-6-phosphate dehydrogenase (G6PD) in blood cells in mice. Moreover, LEH group have higher red blood count, hemoglobin and hematocrit that implied significantly the protection effects on RBC against oxidative stress induced by high fat diet. LEH group also could increase superoxide dismutase (SOD) and G6PD activity in liver of mice. Taken the data of RL or RH groups together, rutin have the same tendency towards antioxidative capacity in blood cells and liver of mice when compared to LES, except for significance level. In conclusion, LES have protection effects on blood cells and liver tissue in mice from oxidative stress induced by high fat diet. The effectiveness of LES relates to the dose of usage, and protection effects against oxidative stress of LES may not only attribute to rutin but also to other bioactive components.
