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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/10432


    Title: 茯苓多醣體應用於化粧品之抗老化分析
    Anti-aging assay of Wolfiporia cocos polysaccharid...
    Authors: 周玟玉
    Min-Yu Chou
    Contributors: 呂敏勇
    嘉南藥理科技大學：化妝品科技研究所
    Keywords: 茯苓
    反應曲面法
    基質金屬蛋白酶
    保濕
    多醣體
    polysaccharides
    Wolfiporia cocos
    response surf
    Date: 2008
    Issue Date: 2010-05-31 13:59:21 (UTC+8)
    Abstract: 本研究的目的在探討茯苓(WolfiPoria cocos)菌絲體液態深層培養生產菌絲體和胞外多醣體(exopolysaccharides)的最佳培養條件；而且我們將萃取獲得的茯苓胞外多醣體處理3T3老鼠纖維母細胞，分析多醣體對基質金屬蛋白酶(matrix metalloproteinases, MMPs)酵素活性及對膠原蛋白含量的影響；並評估其保濕能力。
    以反應曲面法得知含有3%的葡萄糖、0.7%的酵母抽出物和0.7%的玉米抽出物，對茯苓的菌絲體和胞外多醣體可得到最佳的產量；再利用五公升小型醱酵槽進行茯苓菌絲體和胞外多醣體的生產，發現菌絲體會隨著葡萄糖的濃度降低而增加，直到第六天葡萄糖完全被利用殆盡，菌絲體產量可達到9.00 g/L，胞外多醣體的濃度可達到2.93 g/L。
    實驗結果顯示茯苓胞外多醣體對於MMP-2和MMP-9的酵素活性皆無抑制的作用；因此對細胞培養液內所含的膠原蛋白無保護作用。由保濕能力測定的結果顯示，在(1) 3.4%於20分鐘內、(2) 1.5%於15分鐘內、(3) 0.5%於10分鐘內，其保濕能力均比玻尿酸佳；且在60分鐘後保濕能力雖然沒有比玻尿酸來的好，但保濕能力仍維持在94%以上，由實驗數據顯示茯苓胞外多醣體具有良好的保濕幼纂C
    綜合上述的實驗結果，茯苓胞外多醣體在化妝品的應用上可做為天然的保濕成分。
    The purpose of this study was designed to investigate the optimum condition for the production of mycelia and exopolysaccharides (WCEPs) in submerged culture of Wolfiporia cocos. Furthermore, we treated 3T3 mouse fibroblasts with WCEPs, then assaying the effect of WCEPs on the activities of MMPs and the content of collagen present in the culture medium. Additionally, we evaluated the moisturizing abilities of WCEPs.
    The data obtained from response surface methodology showed that the fermentation broth including 3% glucose, 0.7% yeast extract, and 0.7% corn steep powder could give rise to the optimum yield of mycelia and WCEPs. The 5-L fermentors were also employed for the production of mycelia and WCEPs. We found that after 6-day fermentation, glucose was nearly depleted, and the concentrations of W. cocos mycelia and WCEPs in submerged medium were 9.00 g/L and 2.93 g/L, respectively.
    The results also showed that WCEPs could not inhibit the activities of MMP-2 and MMP-9, and therefore, collagen in the present of culture medium was not preserved. On the other hand, we demonstrated that compared with hyaluronans, WCEPs in 3.4%, 1.5%, and 0.5% possessed a superior moisturizing ability within 20 min, 15min, and 10 min, respectively. During 60 min, moisture storage capacity in WCEPs was maintained above 95% at room temperature. The data demonstrated that WCEPs had the exceptional moisturizing efficacy.
    The results presented here suggest that WCEPs can be used as a natural moisturizing agent and applied in skincare cosmetic products.
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