DSpace collection: 期刊論文

Inhibition of lncRNA RPPH1 activity decreases tumor proliferation and metastasis through down-regulation of inflammation-related oncogenes

Wed, 25 Dec 2024 03:05:44 GMT

title: Inhibition of lncRNA RPPH1 activity decreases tumor proliferation and metastasis through down-regulation of inflammation-related oncogenes abstract: Objective: Ribonuclease P RNA component H1 (RPPH1) is a long non-coding RNA (lncRNA) associated with cancer progression. Higher RPPH1 expression in breast and cervical cancer samples than that in normal tissues were observed through the lncRNASNP2 database; therefore, silencing RPPH1 expression might be a potential strategy for cancer treatments, even though RPPH1 is also an RNA subunit of ribonuclease P involved in processing transfer RNA (tRNA) precursors and the effect of RPPH1 knockdown is not yet fully understood. Methods: Differentially expressed genes (DEGs) were identified through RNA sequencing in each shRNA-transfected RPPH1 knockdown MDA-MB-231, RPPH1 knockdown HeLa cell, and respective control cells, then the gene ontology enrichment analysis was performed by IPA and MetaCore database according to these DEGs, with further in vitro experiments validating the effect of RPPH1 silencing in MDA-MB-231 and HeLa cells. Results: Hundreds of down -regulated DEGs were identified in RPPH1 knockdown MDA-MB-231 and HeLa cells while bioinformatics analysis revealed that these genes were involved in pathways related to immune response and cancerogenesis. Compared to mock-and vector-transfected cells, the production of mature tRNAs, cell proliferation and migration capacity were inhibited in RPPH1-silenced HeLa and MDA-MB-231 cells. Additionally, RPPH1 knockdown promoted G1 cell cycle arrest mainly through the down-regulation of cyclin D1, although glycolytic pathways were only affected in RPPH1 knockdown HeLa cells but not MDA-MB-231 cells. Conclusion: This study demonstrated that knockdown RPPH1 affected tRNA production, cell proliferation and metabolism. Our findings might provide insight into the role of RPPH1 in tumor development.

Fabrication of localized surface plasmon resonance sensors with scalable polyvinyltetrazole/copper cluster hybrid ring-array for Cu(II) detection

Wed, 25 Dec 2024 03:04:43 GMT

title: Fabrication of localized surface plasmon resonance sensors with scalable polyvinyltetrazole/copper cluster hybrid ring-array for Cu(II) detection abstract: The bottom of a hole-array photoresist template deposited with a hydrophobic atom-transfer radical polymerization (ATRP) initiator was wetted by treatment with oxygen plasma. After the removal of the photoresist template, ring patterns of the ATRP initiator were formed at the interface between the hydrophobic and wetting regions. Polyacrylonitrile (PAN) was grafted from the initiator ring array to covert to polyvinyltetrazole (PVT) rings via a cyano-to-tetrazole reaction, which could adsorb Cu(II) at various concentrations. The Cu(II) ions within the PVT rings were reduced to form a PVT-copper hybrid ring (VCHR), resulting in a blue-shift of the localized surface plasmon resonance (LSPR) peak as the Cu(II) was adsorbed by the PVT rings. The blue-shift and Cu(II) concentration were linearly correlated, with a detection limit of similar to 25 pg mL(-1) and a linear range of 25-400 pg mL(-1) for Cu(II) detection. Although the PVT rings also chelated Pb(II) and Cr(III), these ions did not exhibit obvious LSPR peaks. The VCHR LSPR sensor exhibited excellent selectivity for Cu(II) detection. Combining lithography and plasma technology provides a versatile platform for developing the scalable ring structure of copper for highly sensitive and selective Cu(II) sensing.

Poly[2-(dimethylamino)ethyl methacrylate]/gold nanoparticle composite dual-stripe nanowire arrays as optical biosensors for label-free plague diagnosis

Wed, 25 Dec 2024 03:04:16 GMT

title: Poly[2-(dimethylamino)ethyl methacrylate]/gold nanoparticle composite dual-stripe nanowire arrays as optical biosensors for label-free plague diagnosis abstract: Background: Most commercial kit provides a meager limit of detection of 10(4) CFU/mL. Antigen determination remains an urgent need for further development of rapid, sensitive, and reliable diagnostic methods for use at the point of need. Methods: Amide-terminated silane, a water immiscible compound before hydrolysis, was evaporated for deposition on the alternative wetting and dewetting regions. The silane compound was hydrolyzed along the interfaces of wetting and dewetting regions to form a dual-stripe of an initiator after halogenation. Poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) was grafted from the dual-stripe of the initiator to attract gold nanoparticles (AuNPs) as AuNP-PDMAEMA composite dual-stripe nanowire (APDW) arrays via alternate phase transition cycles. The APDW arrays were modified with a Yersinia pestis (abY) antibody to detect the antigen of Yersinia pestis (agY) for plague diagnosis. Significant findings: The binding of the agY onto the abY-anchored APDW array resulted in a geometrical change from a dual-stripe to a two-humped structure. The reflectance spectra could be used to analyze the AuNP attachment and the agY binding onto the abY-anchored APDW array. The linear range between the wavelength shift and agY concentration from 20 to 440 pg mL(-1) was established to obtain the detection limit of similar to 14.2 pg mL(-1).

Designable Poly(methacrylic Acid)/Silver Cluster Ring Arrays as Reflectance Spectroscopy-Based Biosensors for Label-Free Plague Diagnosis

Wed, 25 Dec 2024 03:03:56 GMT

title: Designable Poly(methacrylic Acid)/Silver Cluster Ring Arrays as Reflectance Spectroscopy-Based Biosensors for Label-Free Plague Diagnosis abstract: A hole array was fabricated via photolithography to wet the bottoms of holes using oxygen plasma. Amide-terminated silane, a water immiscible compound before hydrolysis, was evaporated for deposition on the plasma-treated hole template surface. The silane compound was hydrolyzed along the edges of circular sides of the hole bottom to form a ring of an initiator after halogenation. Poly(methacrylic acid) (PMAA) was grafted from the ring of the initiator to attract Ag clusters (AgCs) as AgC-PMAA hybrid ring (SPHR) arrays via alternate phase transition cycles. The SPHR arrays were modified with a Yersinia pestis antibody (abY) to detect the antigen of Yersinia pestis (agY) for plague diagnosis. The binding of the agY onto the abY-anchored SPHR array resulted in a geometrical change from a ring to a two-humped structure. The reflectance spectra could be used to analyze the AgC attachment and the agY binding onto the abY-anchored SPHR array. The linear range between the wavelength shift and agY concentration from 30 to 270 pg mL(-1) was established to obtain the detection limit of similar to 12.3 pg mL(-1). Our proposed method provides a novel pathway to efficiently fabricate a ring array with a scale of less than 100 nm, which demonstrates excellent performance in preclinical trials.