DSpace collection: 10期 (1984)
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以Trichoderma Koningii W-10 菌株之纖維素分解酵素糖化竹筍殼及稻殼之研究
http://ir.cnu.edu.tw/handle/310902800/25515
title: 以Trichoderma Koningii W-10 菌株之纖維素分解酵素糖化竹筍殼及稻殼之研究 abstract: 本試驗係採用Trichoderma koningii W-10菌株從事固體培養以生產纖維素分解酵素，所用基質分別為竹筍殼及稻殼。此兩種織維質廢棄物之成分業經分析。於使用之前，二者皆採用4% NaOH溶液，於室溫下前處理24小時後作為培養基之成分及供作糖化之基質。採用4g稻殼，6g麩皮及加入10 ml 0.6% NH4NO3溶液，於30℃之下培養4天，可約獲得每g 16FPu及15 Sau之纖維素分解酵素;而採用6g竹筍殼、 4g麩皮及加入10m 10.9%NH4NO3溶液，於30℃之下培養3天，則可獲得約每g 12.5 FPu及12 Sau之纖維素分解酵素。其它酵素活性包括C1、Cx活性亦分別測定之。此酵素之FPA及β-glucosidase活性之最適反應溫度及pH皆為55℃及4.0。二者之較佳溫度安定性範圍皆低於50℃。FPA之pH安定性範圍由48至6.0β-glucosidase活性之pH安定性範圍則由3.0至6.0。所抽取之粗酵素液於10℃之下保存兩個月，其FPA尚存有91%以上。使用粗酵素液以從事糖化試驗時，發現以每ml含1.5FPu及1.35Sau之粗酵素液於50℃及pH4.0之下進行糖化，其糖化反應液中分別含有20%稻殼或18%竹筍殼，則於第48小時可獲得3 %還原糖之稻殼水解液或6%還原糖之竹筍殼水解液。以濾紙色層分析法檢定水解液中之糖類成分時，發現二者均含有葡萄糖、木糖及少量阿刺伯糖。然而，於竹筍殼水解液中發現一未明色點，可能為五碳寡醣。
<br>Wed, 12 Sep 2012 08:29:31 GMTBenzo-derivatives of mutagenic-carcinogenic 2-aminodipyrido[1,2-a:3',2'-d]imidazole
http://ir.cnu.edu.tw/handle/310902800/25514
title: Benzo-derivatives of mutagenic-carcinogenic 2-aminodipyrido[1,2-a:3',2'-d]imidazole abstract: Benzo-derlvatives of mutaqenic-carcinoqenic 2-aminodipyrido[1,2-a:3 ,2.-d]imidazole (Glu-P-2) were synthesized in short steps. The interaction of the compounds with DNA and their mutagenicity were investigated.
<br>Wed, 12 Sep 2012 08:29:29 GMTThe Implication of Thoracic Duct Lymph in the Distribution and Elimination of Rabbit Muscle Creatine Phosphokinase
http://ir.cnu.edu.tw/handle/310902800/25513
title: The Implication of Thoracic Duct Lymph in the Distribution and Elimination of Rabbit Muscle Creatine Phosphokinase abstract: The implication of thoracic duct Iymph in the distribution and the elimination of rabbit muscle creatine phpsphokinase (CPK) was investigated in situ and in vitro with 30 rabbits. The rate of thoracic duct lymph flow per kg body weight in anesthetized rabbits was 0.97+-0.21ml.h-1.kg-1 (mean +- S.D., n=9). The CPK activity (U) that appeared in thoracic duct lymph after laparotomy (15 cm dissection along the mid-line) was 0.129 +- 0.038 U/h (n = 4) in untreated rabbits and 4.55 +- 2.23 U/h (n=5) in rabbits given intramuscular administration of CPK(1000U/kg body weight). The rate of transfer of CPK from the circulation to the thoracic duct lymph in rabbits after intravenous administration of CPK (1000 U/kg) was 30.0 +- 11.1 U/h (n=5).The inactivation rate constant of CPK in thoracic duct lymph (pH 7.40, 39℃,0.098 +- 0.023 h-1, n= 5) was larger than that in heparinized whole blood. The result implies that CPK may be partly inactivated in the lymph in vivo. The inactivation rate constant of CPK in thoracic duct lymph fluid without lymphocytes (pH 7.40, 0.159 +-0.013h-1, n=3) was much larger than that in the thoracic duct lymph. The calcium conceotration (14.4 +- 0.8l0mg/dl, n=7)and the magnesium concentration (3.93 +- 0.530mg/dl, n=7) in thoracic duct lymph were determined. The possible mechanism involved in the inactivation of CPK in thoracic duct lymph is discussed.
<br>Wed, 12 Sep 2012 08:29:28 GMTThe Effect of Disposition Model on the Estimation of Micro-Rate Constants of Rabbit Muscle Creatine Phosphokinase
http://ir.cnu.edu.tw/handle/310902800/25512
title: The Effect of Disposition Model on the Estimation of Micro-Rate Constants of Rabbit Muscle Creatine Phosphokinase abstract: Mathematic manipulation was performed on the pharmacokinetic equation parameters of CPK after intravenous administration of 300, 500 and 860 U/kg body weight dose in sixteen male rabbits to investigate the effect of disposition model with or without the involvement of inactivation in the Iymphatic system on the micro-rate constants of CPK. The pharmacokinetic equations and the estimation of micro-rate constants were derived for two distinct two-compartment body models. The mean hybrid distribution rate constant (α) and elimination rate constant (β) were 0.630 +- 0.176 h-1(mean +- S.D., n = 16) and 0.106 +- 0.016 h-1, and were independent on the dose size.The mean micro-rate constants for the model involving an apparent first-order inactivation process of CPK in the Iymph were estimated to be 0.092 +- 0.038 h-1 (k10), 0.245 +- 0.061 (k12) and 0.321 +- 0.146 h-1 (k2l). Student t-test showed the difference of the micro-rate constants among the different dose size and between the two models was insignificant due to the relatively large intersubject variability in rabbits. However,the result of kinetic analysis implied the possible involvement of reticuloendothelial system in the elimination of CPK in vivo.
<br>Wed, 12 Sep 2012 08:29:27 GMT