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Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion

Wed, 25 Dec 2024 03:06:02 GMT

title: Punicalagin is cytotoxic to human colon cancer cells by modulating cell proliferation, apoptosis, and invasion abstract: Purpose: The purpose of this study was to explore the anticancer effect of punicalagin, an abundant bioactive tannin compound isolated from Punica granatum L., on three colon cancer cell lines, namely, HCT 116, HT-29, and LoVo.Research Design: Normal and colon cancer cells were treated with different concentrations of punicalagin for different periods.Data Collection and Analysis: Cell viability was measured with a CCK-8 assay. Programmed cell death and invasion were analyzed using an annexin V and cell death kit and a cell invasion analysis kit. The expression of active caspase-3, MMP-2, MMP-9, Snail, and Slug were measured by Western blot.Results: The results of the cell viability analysis showed that punicalagin was cytotoxic to colon cancer cells, but it was not to normal cells in a dose- and time-dependent manner. Additionally, punicalagin induced apoptosis in colon cancer cells (shown by the cumulative percentage of colorectal cancer cells in early and late apoptosis). It was found that caspase-3 activity increased following punicalagin treatment. Western blot results also showed that punicalagin increased the expression of activated caspase-3. In contrast, punicalagin inhibited the invasion of colon cancer cells. Further, treatment of colon cancer cells with punicalagin suppressed the expression of MMP-2, MMP-9, Snail, and Slug.Conclusions: These results showed that the activation of caspase-3 and the inhibition of MMP-2, MMP-9, Snail and Slug were involved in the effects of punicalagin on colon cancer cells.

Attenuation of blue light-induced photo-oxidative stress through inhibition of NF-κB and MAPK signaling pathways, and activation of Nrf2 signaling pathway by djulis and its bioactive compounds

Wed, 25 Dec 2024 03:04:17 GMT

title: Attenuation of blue light-induced photo-oxidative stress through inhibition of NF-κB and MAPK signaling pathways, and activation of Nrf2 signaling pathway by djulis and its bioactive compounds abstract: The aim of this study is to investigate the djulis and its bioactive compounds to prevent blue light (BL)-induced photo-oxidative stress in ARPE-19 cells. After 6 h of BL exposure (peak at 452 nm), ARPE-19 cell viability significantly decreased, but treatment with water extract of djulis (WECF) at 0.1-25 mu g/mL protected the cells from BL-induced phototoxicity. The cytoprotective effect of WECF were associated with a decrease in BL-induced oxidative stress and caspase-3 activity, and an increase in GSH levels and SOD activity. WECF also inhibited the expression of NF-kappa B protein and the MAPK signaling pathway, while upregulated heme oxygenase-1 (HO-1) via the Nrf2-mediated pathway. Moreover, betanin, kaempferol, quercetin and rutin, present in WECF, showed a protective effect against BL-induced photo-oxidative stress at 1-10 mu M. Overall, WECF and its bioactive com-pounds have the potential to protect ARPE-19 cells from BL-induced retina damage, which could have thera-peutic implications for retinal disease.

Inverse Probability of Treatment Weighting in 5-Year Quality-of-Life Comparison among Three Surgical Procedures for Hepatocellular Carcinoma

Wed, 25 Dec 2024 03:03:32 GMT

title: Inverse Probability of Treatment Weighting in 5-Year Quality-of-Life Comparison among Three Surgical Procedures for Hepatocellular Carcinoma abstract: Simple Summary In patients who had undergone resection for hepatocellular carcinoma (HCC), scores for most quality-of-life (QOL) subscales were significantly improved by 6 months after resection; QOL scores then remained stable for the rest of the 5-year period of this study. The QOL improvements after laparoscopic surgery or robotic surgery were much larger than improvements after open surgery. Between the 2nd and 5th year postsurgery, however, QOL improvements were larger in robotic surgery patients compared to laparoscopic surgery patients. This prospective longitudinal cohort study analyzed long-term changes in individual subscales of quality-of-life (QOL) measures and explored whether these changes were related to effective QOL predictors after hepatocellular carcinoma (HCC) surgery. All 520 HCC patients in this study had completed QOL surveys before surgery and at 6 months, 2 years, and 5 years after surgery. Generalized estimating equation models were used to compare the 5-year QOL among the three HCC surgical procedures. The QOL was significantly (p < 0.05) improved at 6 months after HCC surgery but plateaued at 2-5 years after surgery. In postoperative surveys, the effect size was largest in the nausea and vomiting subscales in patients who had received robotic surgery, and the effect size was smallest in the dyspnea subscale in patients who had received open surgery. It revealed the following explanatory variables for postoperative QOL: surgical procedure type, gender, age, hepatitis C, smoking, tumor stage, postoperative recurrence, and preoperative QOL. The comparisons revealed that, when evaluating QOL after HCC surgery, several factors other than the surgery itself should be considered. The analysis results also implied that postoperative quality of life might depend not only on the success of the surgical procedure, but also on preoperative quality of life.

紅藜與其活性成分對LED光源誘發視網膜傷害之保護效應

Sat, 11 May 2024 03:36:25 GMT

title: 紅藜與其活性成分對LED光源誘發視網膜傷害之保護效應 abstract: 國人接觸電子螢幕時間過長，尤其近年來3C產品充斥、螢幕鮮豔、亮度太高、使用時間過長等因素導致容易傷眼。因此，預防眼睛疾病乃為當務之急。其中藉由發掘天然活性物質以保護眼睛，是近年來的研究重點。現今有些廠商已關注到市場需求，惟受限於人力及研發等因素，有科學數據的相關護眼產品並不多。有鑑於此，本計畫「紅藜與其活性成分對LED光源誘發視網膜傷害之保護效應」之研發成果，可作為業界開發護眼機能產品之參考，尤其針對過度使用LED光源造成傷害之機能素材更值得發掘。一旦量產商品化，其市場競爭力將大為可觀。另在學術上可應用在教學與研究，在輔助醫療上也值得應用與參考。