https://ir.cnu.edu.tw/handle/310902800/25265 Search the Channel s https://ir.cnu.edu.tw//simple-search https://ir.cnu.edu.tw/handle/310902800/25277 title: 甜菊葉中甜菊糖萃取條件之研究 abstract: 存在於甜菊(Stevia rebaudiana Bertoni)葉中的甜菊糖，其甜度約為蔗糖甜度的三百倍，且甜味性質優良，極具開發價值。本研究之目的，在於探討不同條件對於甜菊糖萃取效果之影響，以求山最適之萃取操作條件，作為工業化生產之參考。在萃取過程中，使用內徑2.8cm，長度45 cm，具有保溫外套管的玻璃管柱慎裝乾燥甜菊葉，以水進行升流萃取，得到最適萃取溫度為50~55℃，最適溶劑流速為4.5ml/min。以多管柱升流方式萃取甜菊糖，可較單管柱方式減少大量溶劑，有利於隨後蒸發濃縮所需能源之節約，而且可在較短時間內得到較高濃度之甜菊楮萃取液。以二管柱萃取所得之甜菊楮萃取液，其stevioside之濃度為單管柱時(524mg/ml)的1.85倍(9.7 mg/ml)，而所需溶劑體積僅為單管柱時的55%，以三管柱萃取時，則分別為3.05倍(16.0mg/ml)與33%。 <br> https://ir.cnu.edu.tw/handle/310902800/25276 title: The Absorption Kinetic of Rabbit Muscle Creatine Phosphokinase after Intramuscular Administration abstract: 吸收動態的探討是藥物動力學中極為重要但又屬於比較困難的部分。作者曾報告CPK於肌注後之絕對可用率，並以統計學的moment解析法求得其平均吸收時間。然而解析之結果顯示CPK在體內滯留時間之分佈並非對數常態分佈型式，而是偏左的非對稱分佈，使求得之平均吸收時間之意義變成不明確。本報告針對CPK之吸收相以三種不同之數值解纏法(numerical deconvolutioo method)分別解析肌肉注射後CPK之吸收速率過程。CPK之肌肉吸收動態呈二相性零階次動力學特性。0-4h約為53.0土17.0U/b (mean土 S.D., n=5)，而4-12h約為18.1土7.4U /h。傳統上常用之area-area解纏法之結果比數學上較正確之poiot-area解纏法之結果有意的偏高(P<0.05)，而簡化近似值計算法之iostantaneous midpoint-input法之解析結果則顯著地偏低(P<0.05)。CPK之吸收速率在吸收過程中有由快而慢之變化，主要是因為CPK之吸收是經由淋巴系故受到淋巴流速的管制;而淋巴流速是受到組織絀胞間壓及淋巴泵之支配。在肌注後之初期，組織細胞間壓及淋巴泵之活性皆很高，經過一段時間後才能恢復正常，這種變化正反映於CPK之吸收速率之變化。CPK肌注後之平均吸收時間為12h，此結果和前以統計學的moment解析法所得之結果完全相同。然而decoavolution解析法較能闡明吸收過程之變化動態同時只須要較短時間之測定資料，其繁雜之計算可由電子計算機代勞。 <br> https://ir.cnu.edu.tw/handle/310902800/25275 title: Antipyretic Effect of Aspirin-Phenacetin Preparation and Their Plasma Concentrations in Febrile Rats Induced by Canageenin abstract: The effects of carrageenin on the antipyretic activity and the plasma concentration of drugs after oral administration of aspirin-phenacetin preparation in rats were investigated. 1) The reproducible hyperthermic pattern was obtained by administration of carrageenin in rats. There were no remarkable changes in liver function of febrile rats induced by carrageenin. The rectal temperature was rapidly decreased by oral administration of aspirin-phenacetin preparation, but the rectal temperature of normal rats was not influenced. 2) In carrageenin febrile rats, plasma concentrations of aspirin, phenacetin and their metabolites, salicylic acid and acetaminophen, were lower than those in normal rats. It was suggested that these results were due to depression of gastric emptying rate and propulsive rate of intestine. 3) Fatty acids of lipid in the serum from febrile rats decreased and those in the brain increased. After administation of aspirin-phenacetin preparation in febrile rats, the fatty acid composition of lipid in the brain was altered and the amounts of oleic acid and arachidonic acid incresed. 4) The variations in serum electrolytes were observed in carrageenin febrile rats. It was suggested that these variations were related to a decrease in bile flow, a rise in blood sugar value and a decrease in aspirin esterase activity. <br> https://ir.cnu.edu.tw/handle/310902800/25274 title: Simultaneous determination of piroxicam and its main metabolite in plasma and urine by high-performance liquid chromatography abstract: A rapid and sensitive high-performance liquid chromatographic procedure is described for the simultaneous determination of piroxicam and its main metabolite,such as 5-hydroxypiroxicam, in plasma and urine. Acidified plasma (pH 3.0) was extracted with ethyl ether and indomethacin was used as an internal standard. The organic extract was reduced to dryness, the resultant residue redissolved in the mobile phase, and aliquots of this solution chromatographed on a Lichrosorb RP-18(7 μm) column using a mobile phase consisting of an acetonitrile-water-acetic acid(58 : 38 : 4) mixture. The flow rate was 1.2 ml/min and the effluent was monitored at 365 nm with 0.02 aufs. The sensitivities of this method were 0.05μg/ml levels of Piroxicam and 5-hydroxypiroxicam in the plasma and urine samples. <br>