| 摘要: | 多醣體是屬於天然來源物質，大多數來自於細菌、靈芝、植物等，而這些多醣體一般在抗腫瘤、免疫刺激及抑制一些病毒之感染活性方面有釵h研究，因此具有極高的利用價值。 本研究目的是以小球藻（chlorella pyrenoidosa）為材料來萃取純化多醣體在抗氧化方面，當胞內和胞外多醣體濃度於1.5mg/mL時對DPPH自由基清除率分別為70%和16%；在總抗氧化能力（TEAC）測定方面，當胞內多醣體濃度於1.5mg/mL時，相當於0.6mM之trolox量，而胞外多醣體為0.3mM；在脂質過氧化方面，胞內與胞外多醣體濃度於1.5mg/mL時，其抑制脂質過氧化能力分別為63%和10%；在抑菌作用方面，其結果顯示胞內與胞外多醣體（1）針對大腸桿菌之最小抑制濃度（MIC）和最低殺菌濃度（MBC）為125、145mg/mL和125、155mg/mL（2）針對綠膿桿菌之MIC和MBC為125、85 mg/mL和MBC為125、85 mg/mL；（3）胞內多醣體針對痤瘡桿菌之MIC和MBC則為16mg/mL和31mg/mL，胞外多醣則無抑菌作用；在保濕能力測定方面胞內及胞外多醣體在二十分鐘後，皮膚含水量約維持在35%和32%；在抗發炎作用方面，抑制5-脂氧合酶（5-Lipoxygenase）酵素活性的試驗方面，胞內多醣之IC50為0.136mg/mL，而胞外多醣為14.150mg/mL。在抑制基質金屬蛋白酶方面胞內多醣體濃度為3、7mg/mL時，於作用72小時後具有抑制MMP-2的酵素活性幼纂A但於96小時後其抑制MMP-2的酵素活性效果更為明顯。胞外多醣體則無明顯作用。 整體而言，小球藻之多醣體的弁鈰ㄓF已知具有抗腫瘤、增強免疫刺激的活性之外，本研究結果亦顯示其亦具有抗氧化、抗菌、保濕、抗發炎及抗老化效果。
Polysaccharides are natural materials. They are most acquired from bacteria, ganoderma and higher plants. There are many studies of antitumor, immunostimulation and anti-virus have been discovered in the extremely high value of polysaccharides.
This study is designed to investigate the extract and purification of polysaccharides from Chlorella pyrenoidosa. The DPPH radical scavenging activities of the intracellular and extracellular polysaccharides are 70% and 16% for 1.5mg/mL, respectively. Trolox equivalent antioxidant capacity of the intracellular polysaccharides（1.5mg/mL）is 0.6mM and extracellular polysaccharides（1.5mg/mL）is 0.3mM . The inhibitory effect of lipid peroxidation in intracellular and extracellular polysaccharides are 63% and 10% for 1.5mg/mL, respectively. The antibacterial activity of the polysaccharide is also studied, it shows that the minimum inhibition and minimum bactericidal concentrations by intracellular and extracellular polysaccharides are (1) 125, 155 and 125, 155mg/mL respectively for Escherichia coli, (2) 125, 85 and 125, 85 mg/mL respectively for Pseudomonas aeruginosa, but only intracellular polysaccharides can do that for 16 and 31mg/mL for Propionibacterium ances, respectively. To determine the effectiveness and moisturizing ability of intracellular and extracellular polysaccharides, the test people were plastered with them and to detect after 20 minutes. The water content of skin nearly maintains up to 35% and 32%. As for inhibition of 5-Lipoxygenase activity, the IC50 of intracellular and extracellular polysaccharides are 0.10 ad 14 mg/mL, respectively. As for inhibiting the activities of matrix metalloproteinases, the intracellular polysaccharides are able to suppress the activities of MMP-2 at the treated concentration of 3、7mg/mL after 72 hours, but it is more obvious to do that after 96 hours. Then, the extracellular polysaccharides do not have this function.
As a whole, it elucidates that the polysaccharides of Chlorella pyrenoidosa except that of functions on antitumor and immunostimulation, this study also demonstrates that these extracts are capable of antioxidant, antibacterial, moisturizing, anti-inflammation and antiaging effects. |
