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    Title: 製備可拋棄電極之藥物伏安檢測器測定茶鹼及其代謝物
    Use of a Disposable Modified Carbon Paste Electrode for Liquid Chromatography-amperometric Detection of Theophylline and Three Metabolites in Human Serum
    Authors: 王來好
    Contributors: 醫藥化學系
    Keywords: Electrochemical sensor
    Disposable modified carbon paste electrode
    Theophylline and three metabolites
    Date: 2006
    Issue Date: 2008-05-16 11:24:05 (UTC+8)
    Publisher: 台南縣:嘉南藥理科技大學醫藥化學系
    Abstract: Chemically modified electrodes, constructed by incorporating potassium hexachloroplatinate K2PtCl6
    into the graphite power/paraffin oil matrix used to fabricate conventional carbon paste electrodes, were
    shown to catalyze the electrooxidation of purine compounds including theophylline, 3-methylxanthine,
    1,3-dimethyluric acid and 1-methyluric acid; the modifier electrodes display electrocatalytic activity for
    the oxidation of purines at 170 mV (vs Ag/AgCl) when used as sensing electrodes in amperometric detection
    following liquid chromatography. The K2PtCl6 electrodes permitted detection of the purines at similar
    values of applied potential. The limit of detection (LOD) of theophylline, 1-MUA, 3-MXT and 1,3-
    DMUA were 1.1 ng mL-1, 3.2 ng mL-1, 1.0 ng mL-1 and 11 ng mL-1, respectively. The calibration graphs
    were linear for theophylline and metabolites over the range of concentration used (50-3200 ng mL-1).
    Theophylline and its metabolites can easily be separated and analyzed in one run using electrochemical
    sensor chromatography with a methanol  phosphate buffer containing 20 mM di-potassium hydrogen
    phosphate (pH 5.50). Serum samples were collected after the oral administration of black tea from human
    volunteers. Quantitative data for the determination of a micromolar amount of theophylline and its metabolites
    in serum samples are in agreement with data obtained by HPLC-UV.
    Appears in Collections:[Dept. of Medicinal Chemistry] Chna Project

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