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| 標題: | Cytotoxicity and cell imaging of six types of carbon nanodots prepared through carbonization and hydrothermal processing of natural plant materials |
| 作者: | Chen, Yu-Yu
Jiang, Wen-Ping
Chen, Huan-Luen
Huang, Hui-Chi
Huang, Guan-Jhong
Chiang, Hsiu-Mei
Chang, Chang-Cheng
Huang, Cheng-Liang
Juang, Tzong-Yuan |
| 貢獻者: | China Med Univ, Dept Cosmeceut
Asia Univ, Dept Occupat Therapy
Chia Nan Univ Pharm & Sci, Dept Pharm
China Med Univ, Dept Chinese Pharmaceut Sci & Chinese Med Resourc
China Med Univ, Master Program Food & Drug Safety
China Med Univ Hosp, Aesthet Med Ctr
China Med Univ, Sch Med
Natl Chiayi Univ, Dept Appl Chem |
| 關鍵字: | GRAPHENE QUANTUM DOTS
LABEL-FREE DETECTION
GREEN SYNTHESIS
ANTIINFLAMMATORY ACTIVITIES
SILVER NANOPARTICLES
MAHONIA-OIWAKENSIS
FACILE SYNTHESIS
DRUG-DELIVERY
IN-VITRO
NITROGEN |
| 日期: | 2021 |
| 上傳時間: | 2023-11-11 11:51:31 (UTC+8) |
| 出版者: | ROYAL SOC CHEMISTRY |
| 摘要: | In this study we prepared six types of carbon nanodots (CNDs) from natural plant materials - through carbonization of two species of bamboo (Bamboo-I, Bamboo-II) and one type of wood (Wood), and through hydrothermal processing of the stem and root of the herb Mahonia oiwakensis Hayata (MO) and of the agricultural waste of two species of pineapple root (PA, PB). The resulting CNDs were spherical with dimensions on the nanoscale (3-7 nm); furthermore, CND-Bamboo I, CND-Wood, CND-Bamboo II, CND-MO, CND-PA, and CND-PB displayed fluorescence quantum yields of 9.63, 12.34, 0.90, 10.86, 0.35, and 0.71%, respectively. X-ray diffraction revealed that the carbon nanostructures possessed somewhat ordered and disordered lattices, as evidenced by broad signals at values of 2 theta between 20 and 30 degrees. CND-Bamboo I, CND-Wood, and CND-Bamboo II were obtained in yields of 2-3%; CND-MO, CND-PA, and CND-PB were obtained in yields of 17.64, 9.36, and 22.47%, respectively. Cytotoxicity assays for mouse macrophage RAW264.7 cells treated with the six types of CNDs and a commercial sample of Ag nanoparticles (NPs) revealed that each of our CNDs provided a cell viability of 90% at 2000 mu g mL(-1), whereas it was only 20% after treatment with the Ag NPs at 62.5 mu g mL(-1). The six types of CNDs also displayed low cytotoxicity toward human keratinocyte HacaT cells, human MCF-7 breast cancer cells, and HT-29 colon adenocarcinoma cells when treated at 500 mu g mL(-1). Moreover, confocal microscopic cell imaging revealed that the fluorescent CND-Bamboo I particles were located on the MCF-7 cell membrane and inside the cells after treatment for 6 and 24 h, respectively. We have thoroughly investigated the photoluminescence properties and carbon nanostructures of these highly dispersed CNDs. Because of the facile green synthesis of these six types of CNDs and their sourcing from abundant natural plants, herbs, and agriculture waste, these materials provide a cost-effective method, with low cytotoxicity and stable fluorescence, for biolabeling and for developing cell nanocarriers. |
| 關聯: | RSC ADV, v.11, n.27, pp.16661-16674 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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