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| 標題: | Cell suspension culture extract of Eriobotrya japonica attenuates growth and induces apoptosis in prostate cancer cells via targeting SREBP-1/FASN-driven metabolism and AR |
| 作者: | Hsieh, Po-Fan
Jiang, Wen-Ping
Basavaraj, Praveenkumar
Huang, Shih-Yin
Ruangsai, Phakkhathorn
Wu, Jin-Bin
Huang, Guan-Jhong
Huang, Wen-Chin |
| 貢獻者: | China Med Univ, Sch Med, Grad Inst Biomed
China Med Univ Hosp, Dept Urol
China Med Univ, Sch Med
China Med Univ, Coll Chinese Med, Sch Chinese Pharmaceut Sci & Chinese Med Resource
Chia Nan Univ Pharm & Sci, Dept Pharm
Asia Univ, Dept Occupat Therapy
China Med Univ, Sch Med, Int Masters Program Biomed Sci
Nihon Pharmaceut Univ
Asia Univ, Dept Hlth & Nutr Biotechnol |
| 關鍵字: | Eriobotrya japonica
Sterol regulatory element-binding protein-1
Fatty acid synthase
Androgen receptor
Apoptosis |
| 日期: | 2021 |
| 上傳時間: | 2023-11-11 11:44:23 (UTC+8) |
| 出版者: | ELSEVIER GMBH |
| 摘要: | Background: Castration-resistant prostate cancer (CRPC) is one of the main causes of male cancer mortality. There is currently no effective treatment to cure this deadly prostate cancer (PCa) progression. However, recent research showed that activation of lipogenesis leads to CRPC progression. It provides a rationale to target the highly lipogenic activity as a novel and promising therapy against lethal CRPC. Purposes: The present study aims to evaluate the anticancer efficacy and the molecular mechanism of cell suspension culture extract from Eriobotrya japonica (EJCE) in PCa, including CRPC. Methods: Cell growth, migration and invasion analyses were performed by MTT method, a wound healing assay and the transwell method, respectively. Apoptosis was assessed by a flow cytometry-based Annexin V-FITC/PI assay, caspase enzymatic activity and Western blot analyses. Lipogenesis was determined by a Fatty Acid Quantification Kit and an Oil Red O staining. The in vivo experiment was conducted by a xenograft mouse model. Results: PCa cell growth, migration and invasion were significantly affected by EJCE. EJCE decreased expression of sterol regulatory element-binding protein-1 (SREBP-1) and fatty acid synthase (FASN) in PCa cells, two main factors for lipogenesis. By inhibiting SREBP-1/FASN, EJCE reduced the intracellular fatty acid levels and lipid droplet accumulation in PCa. Moreover, EJCE down-regulated the androgen receptor (AR) and prostate-specific antigen (PSA) in PCa cells. Significantly, EJCE exhibited the potential anticancer activity by suppressing the growth and leading to apoptosis of CRPC tumors in a xenograft mouse model. Conclusion: These results reveal a novel therapeutic molecular mechanism of EJCE in PCa. Blockade of SREBP-1/FASN-driven metabolism and AR by EJCE could be employed as a potent opportunity to cure malignant PCa. |
| 關聯: | PHYTOMEDICINE, v.93, 153806 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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