花蓮二號山苦瓜酒精萃取物植化素分析及其對小鼠肌肉細胞 C2C12 汲取葡萄糖的影響

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標題:	花蓮二號山苦瓜酒精萃取物植化素分析及其對小鼠肌肉細胞 C2C12 汲取葡萄糖的影響 The Phytochemical Composition of the Ethanol Extracts Hualien No.2 Momordica charantia Linn. and Effects on Glucose Uptake in C2C12 Myotubes.
作者:	陳秀貞
貢獻者:	保健營養系孫芳明
關鍵字:	The phytochemicals of Momordica charantia Linn. ( M. charantia ) has been the subject of many scientific articles describing their health-promoting properties. Especially, the hypoglycemic and anti-inflammatory active compounds in MCEE and its molecular target have been extensive studied. In this study the phytochemicals composition of the 95 and 50% ethanol extracts of Hualien No.2 Momordica charantia Linn. ( MCEE ) and its effects on glucose uptake in C2C12 myotubes were examined.The total phenolic, flavonoids, saponins, charantin and 10 kda insulin-like Peptide is 6.37 ± 0.99 mg / g GAE, 6.16 ± 0.50 mg / g QE, 179.43 ± 0.87 mg / g, 18.76 ± 0.25 mg / g, and 82.78 ± 7.31 μg /g, respectively. These were no difference among 8 μg/mL MCEE, charantin and150 nM insulin(0.87 μg / ml for differentiated C2C12 myotubes in glucose uptake rate. However, the anti- inflammatory and glucose uptake raising effects of charantin and insulin working better than MCEE for PA treated C2C12 myotubes. The results from this study indicated that phytochemicals ( charantins, saponins, phenonlic compounds and flavonoids ) possess anti- flammatory and glucose uptake raising effects for both differentiated and inflammatory C2C12 myotubes. 山苦瓜 C2C12細胞葡萄糖汲取類胰島素胜肽 Momordica charantia L. C2C12 cells Glucose uptake Insulin like peptides
日期:	2019
上傳時間:	2020-12-09 14:46:02 (UTC+8)
摘要:	山苦瓜 ( Momordica charantia Linn.) 已被證實含有豐富的促進人體健康之植化素，最近研究發現，山苦瓜植化素的降血糖及抗發炎功效是備受關注的，本研究目的為探討花蓮二號山苦瓜以95%乙醇及50%乙醇萃取之酒精萃取物 ( Momordica charantia Ethanol Extract, MCEE )，並測試其總酚、類黃酮、粗蛋白、山苦瓜素、總皂苷及類胰島素胜肽含量，再進行其對正常分化小鼠肌肉細胞 C2C12 和經棕櫚油酸 ( Palmitic acid, PA )誘導發炎C2C12細胞汲取葡萄糖影響。結果顯示，MCEE含6.37 ± 0.99 mg / g GAE總多酚、6.16 ± 0.50 mg / g QE類黃酮、179.43 ± 0.87 mg / g總皂苷、18.76 ± 0.25 mg / g 的山苦瓜素(charantin)、及15.84 ± 0.21 g / 100 g 的粗蛋白，而類胰島素胜肽 ( 10 kda ) 含量約為82.78 ± 7.31 μg / ml； 8 μg/ml的MCEE與charantin對正常分化C2C12細胞與150 nM的胰島素(0.87 μg / ml )具相同的提升葡萄糖吸收效率作用，對於PA誘導產生發炎之C2C12細胞8 μg/ml的MCEE與4 μg/ml的charantin和150 nM的胰島素具有相同的抑制發炎和提升葡萄糖吸收效率作用功能；MCEE對正常或發炎C2C12細胞的葡萄糖汲取和抑制發炎產生胰島素阻抗是有幫助的。
關聯:	電子全文公開日期：2021-08-19 學年度：107，76頁
顯示於類別:	[保健營養系(所) ] 博碩士論文

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