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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32689


    標題: TGR5 activation ameliorates hyperglycemia-induced cardiac hypertrophy in H9c2 cells
    作者: Cheng, Kai-Chun
    Chang, Wei-Ting
    Kuo, Feng Yu
    Zhih-Cherng Chen(陳志成)
    Li, Yingxiao
    Cheng, Juei-Tang
    貢獻者: Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Psychosomat Internal Med
    Chi Mei Med Ctr, Dept Cardiol
    Chi Mei Med Ctr, Dept Med Res
    Vet Gen Hosp, Cardiovasc Ctr
    Chia Nan Univ Pharm & Sci, Dept Pharm
    關鍵字: INDUCED CARDIOMYOCYTE APOPTOSIS
    ENDOPLASMIC-RETICULUM STRESS
    BILE-ACIDS
    SIGNALING PATHWAYS
    RECEPTOR
    INHIBITION
    MECHANISMS
    RESPONSES
    MYOCYTES
    AGONISTS
    日期: 2019-03
    上傳時間: 2020-07-29 13:55:23 (UTC+8)
    出版者: NATURE PUBLISHING GROUP
    摘要: Left ventricular hypertrophy is an independent risk factor in diabetic patients. TGR5 is shown to express in hearts, but its functional role in diabetes-induced cardiac hypertrophy remained unclear. The current study investigated the role of TGR5 on high glucose-induced hypertrophy of H9C2 cells. After incubation with a high level of glucose, H9C2 cells showed hypertrophic responses. Activation of TGR5 by lithocholic acid (LCA) ameliorated cell hypertrophy and enhanced SERCA2a and phosphorylated phospholamban (PLN) expression in H9C2 cells. Triamterene inhibited these effects at an effective dose to block TGR5. However, LCA failed to modify the free radical elevation induced by high-glucose in the H9c2 cells. Moreover, PKA inhibitors, but not an Epac blocker, markedly improved hyperglycemia-induced hypertrophy and attenuated the increased SERCA2a expression by LCA; it also attenuated the phosphorylated PLN and SERCA2a protein expression levels in high glucose-treated H9C2 cells. In conclusion, TGR5 activation stimulated protein kinase A (PKA) to enhance PLN phosphorylation, which activated SERCA2a to remove Ca2+ from cytosol to sarcoplasmic reticulum in addition to the reduction of calcineurin/NFAT pathway signaling to ameliorate the hyperglycemia-induced cardiac hypertrophy shown in cardiomyocytes. TGR5 may service as a new target in the control of diabetic cardiomyopathy.
    關聯: Scientific Reports, v.9, 3633
    Appears in Collections:[藥學系(所)] 期刊論文

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