Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/32506
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    Title: The Microbiological Characteristics of Carbapenem-Resistant Enterobacteriaceae Carrying the mcr-1 Gene
    Authors: Chih-Wei Chen(陳志偉)
    Tang, Hung-Jen
    Chen, Chi-Chung
    Lu, Ying-Chen
    Chen, Hung-Jui
    Su, Bo-An
    Weng, Tzu-Chieh
    Chuang, Yin-Ching
    Lai, Chih-Cheng
    Contributors: Chi Mei Med Ctr, Dept Surg, Div Neurosurg
    Chia Nan Univ Pharm & Sci, Coll Sustainable Environm, Dept Occupat Safety & Hlth, Inst Ind Safety & Disaster Prevent
    Chi Mei Med Ctr, Dept Med
    Chi Mei Med Ctr, Dept Med Res
    Natl Chiayi Univ, Dept Food Sci
    Chi Mei Med Ctr, Dept Med, Tainan 736, Taiwan
    Chi Mei Med Ctr, Dept Intens Care Med, Tainan 736, Taiwan
    Keywords: antibiotic resistance
    carbapenem-resistant Enterobacteriaceae
    colistin
    Escherichia coli
    mcr-1
    Date: 2019-02
    Issue Date: 2020-07-29 13:47:47 (UTC+8)
    Publisher: MDPI
    Abstract: Objectives: This study aims to assess the prevalence of the mcr-1 gene among carbapenem-resistant Enterobacteriaceae (CRE) isolated from clinical specimens and to further investigate the clinical significance and microbiological characteristics of CRE carrying the mcr-1 gene. Methods: Four hundred and twenty-three CRE isolates were screened for the presence of the mcr-1 gene. After identification, their clinical significance, antibiotic susceptibility, and antibiotic resistance mechanisms including the ESBL gene, carbapenemase gene, outer membrane protein (OMP), and plasmid sequencing were assessed. Results: Only four (0.9%) isolates of carbapenem-resistant Escherichia coli (E. coli) were found to carry the mcr-1 gene and demonstrated different pulsed-field gel electrophoresis (PFGE) patterns and sequence types (ST). While one patient was considered as having mcr-1-positive carbapenem-resistant E. coli (CREC) colonization, the other three mcr-1-positive CREC-related infections were classified as nosocomial infections. Only amikacin and tigecycline showed good in vitro activity against these four isolates, and three of them had a minimum inhibitory concentration with colistin of >= 4 mg/L. In the colistin-susceptible isolate, mcr-1 was nonfunctional due to the insertion of another gene. In addition, all of the mcr-1-positive CREC contained various resistant genes, such as AmpC(CMY), bla(NDM), bla(TEM), bla(SHV), and bla(CTX). In addition, one strain (EC1037) had loss of the OMP. Conclusions: The emergence of the mcr-1 gene among CRE, especially E. coli, remains worth our attention due to its resistance to most antibiotics, and a further national survey is warranted.
    Relation: Journal of Clinical Medicine, v.8, n.2, 261
    Appears in Collections:[Dept. of Occupational Safety] Periodical Articles

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