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    標題: Antioxidant and Lipoxygenase Inhibitory Activity of the Kino of Eucalyptus citriodora
    作者: Hung, W. J.
    Chen, Z. T.
    Lee, S. W.
    貢獻者: Chia Nan Univ Pharm & Sci, Dept Med Chem
    Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
    Chia Nan Univ Pharm & Sci, Inst Cosmet Sci
    關鍵字: Eucalyptus citriodora
    kino
    antioxidant
    phenolic content
    flavonoid content
    lipoxygenase
    日期: 2018-09
    上傳時間: 2019-12-16 09:35:06 (UTC+8)
    出版者: WOLTERS KLUWER MEDKNOW PUBLICATIONS
    摘要: In the present study, antioxidant activity, total phenolic and flavonoid content, and 15-lipoxygenase inhibitory activity of ethanol extract and its ethyl acetate, n-butanol and aqueous fractions of Eucalyptus citriodora kino were evaluated. The antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods. The IC50 values of the ethanol extract were 5.11 +/- 0.13, 2.72 +/- 0.08 and 25.86 +/- 1.81 mu g/ml in the 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) methods and the 15-lipoxugenase assay, respectively. Total phenolic and flavonoid content of the ethanol extract were 490.77 +/- 1.95 mg catechin equivalents/g and 21.81 +/- 0.23 mg quercetin equivalents/g, respectively. Solvent partition of the ethanol extract yielded ethyl acetate, n-butanol and aqueous fractions. Among the three fractions, the ethyl acetate fraction showed the highest total phenolic and flavonoid contents, which were 575.87 +/- 3.92 mg catechin equivalents/g and 34.57 +/- 0.30 mg quercetin equivalents/g, respectively. This fraction also showed the strongest free radical scavenging activity in the two methods used as well as inhibitory activity against 15-lipoxygenase, with IC50 values of 4.67 +/- 0.09, 2.57 +/- 0.06 and 14.67 +/- 0.93 mu g/ml, respectively. These findings revealed that high antioxidant and lipoxygenase inhibitory activity of Eucalyptus citriodora kino might be due to high phenolic and flavonoid content. These results showed that Eucalyptus citriodora kino could be a potential source of natural antioxidants and lipoxygenase inhibitors which could be used to prevent free radical and lipoxygenase mediated diseases.
    link: http://dx.doi.org/10.4172/pharmaceutical-sciences.1000444
    關聯: Indian Journal of Pharma Ceutical Sciences, v.80, n.5, pp.955-959
    顯示於類別:[化妝品應用與管理系(所)] 期刊論文
    [食藥產業暨檢測科技系(含五專)] 期刊論文

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