English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16984/19280 (88%)
Visitors : 8692832      Online Users : 672
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32299


    標題: Simvastatin induces G(1) arrest by up-regulating GSK3 and down-regulating CDK4/cyclin D1 and CDK2/cyclin E1 in human primary colorectal cancer cells
    作者: Chen, Ming-Jenn
    Cheng, An-Ching
    Lee, Ming-Fen
    Hsu, Yi-Chiang
    貢獻者: Chi Mei Med Ctr, Dept Surg
    Chia Nan Univ Pharm & Sci, Dept Sports Management, Coll Leisure & Recreat Management
    Chang Jung Christian Univ, Dept Nutr & Hlth Sci, 1 Changda Rd Gueiren Dist
    Chang Jung Christian Univ, Dept Med Sci Ind, Coll Hlth Sci, 1 Changda Rd Gueiren Dist
    關鍵字: colorectal cancer
    cyclin D1
    G(1) arrest
    glycogen synthase kinase 3
    simvastatin
    日期: 2018-06
    上傳時間: 2019-11-15 15:48:40 (UTC+8)
    出版者: WILEY
    摘要: Simvastatin (SIM), a widely used cholesterol-lowering drug, also exhibits tumor-suppressive potentials in several types of malignancy. Colorectal cancer (CRC), the third most common malignant neoplasm, accounts for the second most leading cause of cancer-related deaths worldwide. In the present study, we investigated the anticancer effects of SIM on CRC using primary cancer cells lines (CPs: CP1 to CP5) isolated from five Taiwanese colorectal cancer patients as a model for colorectal cancer. We treated all five CPs with SIM for 24-72hr and observed the respective cell viability by an MTT assay. SIM increased DNA content of the G(1) phase, but did not induce apoptosis/necrosis in CPs as shown by flow cytometry with propidium iodide (PI)/annexin V double staining and PI staining. The expression of G(1) phase-related proteins was analyzed by RT-PCR and Western blotting. SIM suppressed cell growth and induced cell cycle G(1)-arrest by suppressing the expression of CDK4/cyclin D1 and CDK2/cyclin E1, but elevating the expression of glycogen synthase kinase 3 in CPs. Our findings indicate that SIM may have antitumor activity in established colorectal cancer.
    link: http://dx.doi.org/10.1002/jcp.26156
    Appears in Collections:[運動管理系] 期刊論文

    Files in This Item:

    File Description SizeFormat
    10.1002-jcp.26156.pdf3165KbAdobe PDF12View/Open
    index.html0KbHTML20View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback