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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32294


    標題: LRWD1 Regulates Microtubule Nucleation and Proper Cell Cycle Progression in the Human Testicular Embryonic Carcinoma Cells
    作者: Wang, Chia-Yih
    Hong, Yu-Han
    Syu, Jhih-Siang
    Tsai, Yung-Chieh
    Liu, Xiu-Ying
    Chen, Ting-Yu
    Su, Yin-Mei
    Kuo, Pao-Lin
    Lin, Yung-Ming
    Teng, Yen-Ni
    貢獻者: Natl Cheng Kung Univ, Dept Cell Biol & Anat, Coll Med
    Natl Cheng Kung Univ, Inst Basic Med Sci, Coll Med
    Natl Univ Tainan, Dept Biol Sci & Technol
    Chi Mei Med Ctr, Dept Obstet & Gynecol
    Taipei Med Univ, Dept Med
    Chia Nan Univ Pharm & Sci, Dept Sport Management
    Natl Cheng Kung Univ, Dept Obstet & Gynecol, Coll Med
    Natl Cheng Kung Univ, Dept Urol, Coll Med
    關鍵字: LRWD1
    CENTROSOME
    CELL CYCLE
    MICROTUBULE NUCLEATION
    AUTOPHAGY
    日期: 2018-01
    上傳時間: 2019-11-15 15:48:29 (UTC+8)
    出版者: WILEY
    摘要: Leucine-rich repeats and WD repeat domain containing protein 1 (LRWD1) is a testis-specific protein that mainly expressed in the sperm neck where centrosome is located. By using microarray analysis, LRWD1 is identified as a putative gene that involved in spermatogenesis. However, its role in human male germ cell development has not been extensively studied. When checking in the semen of patients with asthenozoospermia, teratozoospermia, and asthenoteratozoospermia, the level of LRWD1 in the sperm neck was significantly reduced with a defective neck or tail. When checking the sub-cellular localization of LRWD1 in the cells, we found that LRWD1 resided in the centrosome and its centrosomal residency was independent of microtubule transportation in NT2/D1, the human testicular embryonic carcinoma, cell line. Depletion of LRWD1 did not induce centrosome re-duplication but inhibited microtubule nucleation. In addition, the G1 arrest were observed in LRWD1 deficient NT2/D1 cells. Upon LRWD1 depletion, the levels of cyclin E, A, and phosphorylated CDK2, were reduced. Overexpression of LRWD1 promoted cell proliferation in NT2/D1, HeLa, and 239T cell lines. In addition, we also observed that autophagy was activated in LRWD1 deficient cells and inhibition of autophagy by chloroquine or bafilomycin A1 promoted cell death when LRWD1 was depleted. Thus, we found a novel function of LRWD1 in controlling microtubule nucleation and cell cycle progression in the human testicular embryonic carcinoma cells. J. Cell. Biochem. 119: 314-326, 2018. (c) 2017 Wiley Periodicals, Inc.
    link: http://dx.doi.org/10.1002/jcb.26180
    關聯: Journal of Cellular Biochemistry, v.119, n.1, pp.314-326
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