English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18033/20233 (89%)
造訪人次 : 23242638      線上人數 : 320
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/32222


    標題: In Vitro and In Vivo Antitumor Effects of Pyrimethamine on Non-small Cell Lung Cancers
    作者: Lin, Meng-Xian
    Lin, Sheng-Hao
    Lin, Chi-Chen
    Yang, Ching-Chieh
    Yuan, Sheau-Yun
    貢獻者: Natl Chung Hsing Univ, Inst Biomed Sci
    Changhua Christian Hosp, Dept Internal Med, Div Chest Med
    Chi Mei Med Ctr, Dept Radiat Oncol
    Natl Sun Yat Sen Univ, Inst Biomed Sci
    Chia Nan Univ Pharm & Sci, Dept Pharm
    Taichung Vet Gen Hosp, Dept Med Res
    Hungkuang Univ, Dept Nursing
    關鍵字: Non-small cell lung cancer (NSCLC)
    pyrimethamine
    cell cycle
    apoptosis
    mitochondrial damage
    日期: 2018-06
    上傳時間: 2019-11-15 15:45:44 (UTC+8)
    出版者: INT INST ANTICANCER RESEARCH
    摘要: Background/Aim: Pyrimethamine (PYR), an antimalarial drug is known to inhibit various types of human cancer cells. The aim of this study was to investigate the anti-tumour effects of pyrimethamine (PYR) and its underlying molecular mechanisms using the human NSCLC cell line A549. Materials and Methods: PYR was dissolved in dimethyl sulfoxide to determine its apoptotic activity on A549 cells. Cell viability was determined by the MTT assay. Cell cycle, mitochondrial membrane potential, and Annexin V-FITC early apoptosis detection were evaluated by flow cytometry. Cyclin-dependent kinase (CDK) and Bcl-2 family protein expression was determined by western blotting. Results: PYR reduced cell viability percentage and induced G(0)/G(1) arrest, which was associated with down-regulation of cyclins D1 and E, CDK4, and CDK2, and up-regulation of p21. PYR induced sub-G(1) accumulation, Annexin-V binding, caspase-9 and -3 activation, poly (ADPribose) polymerase cleavage, and mitochondrial dysfunction in A549 cells. Moreover, PYR effectively inhibited NSCLC tumour growth in an A549 xenograft model. Conclusion: PYR demonstrated anti-tumour effects on NSCLC in vitro and in vivo, indicating its therapeutic potential against human NSCLC.
    link: http://dx.doi.org/10.21873/anticanres.12612
    關聯: Anticancer Research, v.38, n.6, pp.3435-3445
    顯示於類別:[藥學系(所)] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML1182檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋