The impact of inoculum size on the activity of cefoperazone-sulbactam against multidrug resistant organisms

doi:10.1016/j.jmii.2017.08.026

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/32208

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Title:	The impact of inoculum size on the activity of cefoperazone-sulbactam against multidrug resistant organisms
Authors:	Chang, Ping-Chin Chen, Chi-Chung Lu, Ying Chen Lai, Chih-Cheng Huang, Hui-Ling Chuang, Yin-Ching Tang, Hung-Jen
Contributors:	Chi Mei Med Ctr, Dept Internal Med Chi Mei Med Ctr, Dept Med Res Natl Chiayi Univ, Dept Food Sci Chi Mei Med Ctr, Dept Intens Care Med Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chi Mei Med Ctr, Dept Med
Keywords:	Cefoperazone-sulbactam Extended-spectrum beta-lactamases Escherichia coli Klebsiella pneumoniae Inoculum size
Date:	2018-04
Issue Date:	2019-11-15 15:45:14 (UTC+8)
Publisher:	ELSEVIER TAIWAN
Abstract:	Objectives: This study aims to assess the in vitro activity of cefoperazone alone and different cefoperazone-sulbactam ratios against different inoculum sizes of multidrug resistant organisms. Methods: Minimum inhibitory concentrations (MICs) of cefoperazone, cefoperazone-sulbactam at fixed ratio of 1: 1 and 2: 1 against a normal inoculum size of 5 x 10(5) CFU/ml and a high inoculum size of 5 x 10(7) CFU/ml were measured. Results: Each 33 isolates of extended-spectrum beta-lactamases (ESBL)-producing Escherichia coli, ESBL-producing Klebsiella pneumoniae, carbapenem-resistant E. coli, and carbapenem-resistant Pseudomonas aeruginosa and a total of 122 isolates of carbapenem-resistant Acinetobacter baumannii were collected. After the addition of sulbactam at a 1: 1 ratio, most MIC50 and MIC90 values decreased. Cefoperazone-sulbactam at a 1: 1 ratio had a higher susceptibility rate against ESBL-producing E. coli, carbapenem-resistant E. coli, and carbapenem-resistant A. baumannii than cefoperazone-sulbactam at a 2: 1 ratio (all P < 0.05). For ESBL-producing E. coli, the susceptibility rate of cefoperazone-sulbactam at ratios of (1: 1) and (2: 1) decreased from 97.0 to 87.9% and 90.9 to 60.6%, for normal to high inoculum, respectively. For ESBL-producing K. pneumoniae, both susceptibility rate of cefoperazone-sulbactam at ratios of (1: 1) and (2: 1) decreased from 75.8%, and 63.6% at normal inoculum to 51.5% and 42.4% at high inoculum. Conclusions: Cefoperazone-sulbactam at a 1: 1 ratio has greater in vitro activity against most multidrug resistant organisms than cefoperazone-sulbactam at a 2: 1 ratio. Such combinations were not influenced by the inoculum size of ESBL-producing E. coli and K. pneumoniae and could be a therapeutic option for treating severe infections. Copyright (C) 2017, Taiwan Society of Microbiology. Published by Elsevier Taiwan LLC.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1016/j.jmii.2017.08.026
Relation:	Journal of Microbiology Immunology and Infection, v.51, n.2, pp.207-213
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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