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    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/31929


    標題: 韶子屬萃取物之生物活性評估與保養品應用
    The Bio-activities Evaluation and Cosmetic Products Application of Nephelium sp. Extracts
    作者: 蔡其璋
    貢獻者: 化粧品應用與管理系
    梁家華
    關鍵字: 韶子屬
    Nephelium
    日期: 2018
    上傳時間: 2019-02-27 16:48:52 (UTC+8)
    摘要: 本研究以NL的皮(pericarps)酒萃物(NLPE)、NL皮水萃物(NLPW)、NL果實(fruit)酒萃物(NLFE)、NL果實水萃物(NLFW)、NL籽(seed)酒萃物(NLSE)、NL籽水萃物(NLSW)、NL葉(leaf)酒萃物(NLLE)、NL葉水萃物(NLLW)進行抗氧化、美白、抗發炎、預防光老化及DNA修復之機制探討。
    結果顯示NLPE、NLPW、NLLE和NLLW與其它部位萃取物比,具有較好之清除DPPH·自由基(1,1-diphenyl-2-picrylhydrazyl)和ABTS·+ (2,2'-azino-bis[3-ethyl benzthiazoline-6-sulfonic acid])自由基能力;在還原力與抑制脂質過氧化試驗之效能與對照組抗壞血酸(ascorbic acid, AA)相近,且效能都高於NLFE、NLFW、NLSE和NLSW。而在抑制酪胺酸酶試驗中亦發現NLPE、NLPW、NLLE和NLLW有較高的抑制率,而在總多酚、總黃酮及總花青素含量試驗中,NLPE、NLPW、NLLE和NLLW之含量亦高於NLFE、NLFW、NLSE和NLSW。
    細胞內抗氧化試驗中NLPE、NLPW、NLLE及NLLW可有效抑制皮膚角質株化HaCaT細胞內活性氧物質(reactive oxygen species, ROS)與超氧陰離子(superoxide, O2.-)生成量,並提高穀胱甘肽(reduced glutathione, GSH)含量,且NLPE中主成分TC1亦有相同效果;酵素性抗氧化系統超氧歧化酶(superoxide dismutase, SOD)、榖胱甘肽過氧化酶(glutathione peroxidase, GSHPx)和過氧化氫酶(catalase)之表現亦有上升趨勢。美白試驗利用B16F10細胞進行,結果顯示NLPE與NLLE具有抑制酪胺酸酶、黑色素、多巴氧化酶及環磷酸腺苷(cyclic AMP, cAMP)之活性,且降低黑色素生合成相關蛋白質轉錄因子MITF (microphthalmia-associated transcription factor)、MC1R (melanocortin-1 receptor)、tyrosinase、TRP-2 (tyrosinase-related protein-2)和TRP-1表現。抗發炎效能評估是利用SKH-1小鼠與HaCaT細胞進行試驗,發現NLPE、NLLE與TC1均具降低發炎相關因子腫瘤壞死因子-α (tumor necrosis factor-α, TNF-α)、介白素1β (interleukin-1β, IL-1β)和介白素6 (IL-6)的表現,並使MAPK (mitogen-activated protein kinase)家族的活化降低。預防光老化試驗利用免疫組織化學染色法證實NLPE、TC1與NLLE均能促進膠原增生,並降低彈性纖維的異常堆積,透過3T3L1細胞證實具降低基質金屬蛋白酶(matrix metalloproteinase, MMPs)生成的能力。利用HaCaT細胞證實NLPE、TC1與NLLE能降低經UVB誘發所產生之光化產物環丁烷嘧啶二聚體(cyclobutane pyrimidine dimers, CPDs)和6-4二聚體(6-4 photoproducts, 6-4PPs),進而促使DNA修復。且NLPE、TC1與NLLE是藉由調控核苷酸切除修復機制(nucleotide excision repair, NER)相關蛋白質,來修復經UVB誘發所造成的DNA損傷。
    This study evaluated anti-oxidation, anti-melanogenesis, anti-inflammatory, anti-photoaging and DNA repair properties of ethanol and water extrects from NL pericarps (NLPE, NLPW), fruit (NLFE, NLFW), seed (NLSE, NLSW) and leaf (NLLE, NLLW), respectively.
    The results showed that NLPE, NLPW, NLLE and NLLW had excellent abilities on DPPH? and ABTS?+ free radicals scavenging. The efficacy in reducing power and lipid peroxidation inhibition was similar to the ascorbic acid (AA) group, and the efficacy was higher than those of NLFE, NLFW, NLSE and NLSW. In the inhibition of the mushroom tyrosinase ability, NLPE, NLPW, NLLE and NLLW were also have higher inhibition. The total phenolic content (TPC), total flavonoids content (TFC) and total anthocyanin content (TAC) of NLPE, NLPW, NLLE and NLLW are also higher than those of NLFE, NLFW, NLSE and NLSW. NLPE, NLPW, NLLE, NLLW and TC1 also decreased cellular oxidative stress, increased intracellular GSH content and enhanced the antioxidant enzyme activities, such as superoxide dismutase (SOD), catalaseand glutathione peroxidase (GPx). The antimelanogenesis assay was analyzed by measuring tyrosinase activities, melanin contents, dopaoxidase and cyclin AMP activities. NLPE, NLLE and TC1 also decreased the melanogenesis related protein expression of melanocortin-1 receptor (MC1R), microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein-2 (TRP-2) and tyrosinase-related protein-1 (TRP-1). The anti-inflammatory efficacy evaluation was determined by using SKH-1 mice and HaCaT cells. It was found that NLPE, NLLE and TC1 have inhibited inflammatory factors (TNF-α, IL-1β and IL-6), and decreased activation of the MAPK (mitogen-activated protein kinase). Anti-photoaging assay determined by immunohistochemical staining to confirm that NLPE, NLLE and TC1 can promote collagen proliferation, and reduce the abnormal accumulation of elastic fiber. It was shown to have the ability to reduce the production of matrix metalloproteinases (MMPs). Moreover, we also assessed that NLPE, NLLE and TC1 whether protected DNA from UVB and oxidants damage. NLPE, NLLE and TC1 inhibited activities of DNA fragmentation, UVB photoproducts, such as cyclobutane pyrimidine dimer (CPD) and 6-4 pyrimidine-pyrimidone photoproducts (6-4PPs) formation. It also regulated protein expression of the nuclear excision repair (NER) system. This result indicates that NLPE, NLLE and TC1 regulated protein expression of the NER system and reduced UVB induced DNA damage.
    關聯: 電子全文公開日期:2023-07-30,學年度:106, 84頁
    顯示於類別:[化妝品應用與管理系(所)] 博碩士論文

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