The Dietary Furocoumarin Imperatorin Increases Plasma GLP-1 Levels in Type 1-Like Diabetic Rats

doi:10.3390/nu9111192

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Title:	The Dietary Furocoumarin Imperatorin Increases Plasma GLP-1 Levels in Type 1-Like Diabetic Rats
Authors:	Wang, Lin-Yu Cheng, Kai-Chun Li, Yingxiao Niu, Chiang-Shan Cheng, Juei-Tang Niu, Ho-Shan
Contributors:	Chia Nan Univ Pharm & Sci, Dept Childhood Educ & Nursery Chi Mei Med Ctr, Div Pediat Kaohsiung Med Univ, Coll Med Kagoshima Univ, Grad Sch Med & Dent Sci, Dept Psychosomat Internal Med Chi Mei Med Ctr, Dept Med Res Tzu Chi Univ Sci & Technol, Dept Nursing Chang Jung Christian Univ, Coll Hlth Sci, Inst Med Sci
Keywords:	imperatorin triamterene GPR119 TGR5 transfection sitagliptin
Date:	2017-11
Issue Date:	2018-11-30 15:54:50 (UTC+8)
Publisher:	Mdpi Ag
Abstract:	Imperatorin, a dietary furocoumarin, is found not only in medicinal plants, but also in popular culinary herbs, such as parsley and fennel. Recently, imperatorin has been shown to activate GPR119 in cells. Another GPR, GPR131, also called TGR5 or G-protein-coupled bile acid receptor 1 (GPBAR1), is known to regulate glucose metabolism. Additionally, TGR5 activation increases glucagon-like peptide (GLP-1) secretion to lower blood sugar levels in animals. Therefore, the present study aims to determine whether the effects of imperatorin on GLP-1 secretion are mediated by TGR5. First, we transfected cultured Chinese hamster ovary cells (CHO-K1 cells) with the TGR5 gene. Glucose uptake was confirmed in the transfected cells using a fluorescent indicator. Moreover, NCI-H716 cells, which secrete GLP-1, were used to investigate the changes in calcium concentrations and GLP-1 levels. In addition, streptozotocin (STZ)-induced type 1-like diabetic rats were used to identify the effects of imperatorin in vivo. Imperatorin dose-dependently increased glucose uptake in CHO-K1 cells expressing TGR5. In STZ diabetic rats, similar to the results in NCI-H716 cells, imperatorin induced a marked increase of GLP-1 secretion that was reduced, but not totally abolished, by a dose of triamterene that inhibited TGR5. Moreover, increases in GLP-1 secretion induced by imperatorin and GPR119 activation were shown in NCI-H716 cells. We demonstrated that imperatorin induced GLP-1 secretion via activating TGR5 and GPR119. Therefore, imperatorin shall be considered as a TGR5 and GPR119 agonist.
Relation:	Nutrients, v.9, n.11, pp.1192-
Appears in Collections:	[Dept. of Childhood Education and Nursery] Periodical Articles

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