English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 16354/18762 (87%)
造訪人次 : 5262823      線上人數 : 61
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: http://ir.cnu.edu.tw/handle/310902800/31729


    標題: Skin Delivery of Clec4a Small Hairpin RNA Elicited an Effective Antitumor Response by Enhancing CD8(+) Immunity In Vivo
    作者: Weng, Tzu-Yang
    Li, Chia-Jung
    Li, Chung-Yen
    Hung, Yu-Hsuan
    Yen, Meng-Chi
    Chang, Yu-Wei
    Chen, Yu-Hung
    Chen, Yi-Ling
    Hsu, Hui-Ping
    Chang, Jang-Yang
    Lai, Ming-Derg
    貢獻者: Natl Cheng Kung Univ, Coll Med, Dept Biochem & Mol Biol
    Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci
    Kaohsiung Med Univ,Kaohsiung Med Univ Hosp, Dept Emergency Med
    Natl Cheng Kung Univ, Dept Med
    Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
    Natl Cheng Kung Univ, Coll Med,Natl Cheng Kung Univ Hosp, Dept Surg
    Natl Hlth Res Inst, Natl Inst Canc Res
    關鍵字: Human Dendritic Cells
    Cancer-Immunotherapy
    Lectin Receptors
    Dna Vaccine
    T-Cells
    Indoleamine 2,3-Dioxygenase
    Inhibitory Motif
    Dcir
    Antigen
    Immunoreceptor
    日期: 2017-12-15
    上傳時間: 2018-11-30 15:54:30 (UTC+8)
    出版者: Cell Press
    摘要: Clec4a has been reported to be an immune suppressor of dendritic cells (DCs), but its potential role in cancer therapy remains to be elucidated. The present study investigated whether downregulating the expression of Clec4a via skin delivery of small hairpin RNA (shRNA) using a gene gun produced stronger host immunity and inhibited tumor progression in animal models. Administration of Clec4a2 shRNA delayed tumor growth in both mouse bladder and lung tumor-bearing mouse models. The result was further confirmed with a compensation experiment showing that the antitumor effects induced by Clec4a2 shRNA were restored by co-injection of a plasmid expressing exogenous Clec4a2. Increased numbers of infiltrating CD4(+) and CD8(+) T cells at tumor sites were observed in mice treated with Clec4a2 shRNA. Splenocytes from mice with Clec4a2 shRNA administration exhibited stronger cytotoxic activity compared with splenocytes from control mice. CD8-deletion in vivo abrogated the antitumor effects elicited by Clec4a2 shRNA. Additionally, shClec4a enhanced the antitumor effects of the Neu DNA vaccine in the MBT-2 tumor model. In summary, the findings provide evidence that silencing of Clec4a2 expression via skin delivery of shRNA produces an effective antitumor response and that Clec4a2 shRNA may have therapeutic potential as an adjuvant for cancer immunotherapy.
    關聯: Molecular Therapy-Nucleic Acids, v.9, pp.419-427
    顯示於類別:[老人服務事業管理系] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    10.1016-j.omtn.2017.10.015.pdf1788KbAdobe PDF0檢視/開啟
    index.html0KbHTML10檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋