Antiproliferative effect of sweet orange peel and its bioactive compounds against human hepatoma cells, in vitro and in vivo

doi:10.1016/j.jff.2017.03.051

CNU IR > College of Sustainable Environment and Recreation > Dept. of Recreation and Health-Care Management > Periodical Articles > Item 310902800/31698

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Title:	Antiproliferative effect of sweet orange peel and its bioactive compounds against human hepatoma cells, in vitro and in vivo
Authors:	Chu, Chin-Chen Chen, Shih-Ying Chyau, Charng-Cherng Duh, Pin-Der
Contributors:	Chi Mei Med Ctr, Dept Anesthesiol Chia Nan Univ Pharm & Sci, Dept Recreat & Hlth Care Management Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Hungkuang Univ, Res Inst Biotechnol Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol
Keywords:	Sweet orange peel Nobiletin Tangeretin Human hepatomacellular carcinoma (HCC) HepG2 cells Reactive oxygen species Apoptosis
Date:	2017-06
Issue Date:	2018-11-30 15:53:19 (UTC+8)
Publisher:	Elsevier Science Bv
Abstract:	The ethyl acetate extracts from sweet orange peel (EESP) and its bioactive compounds against human hepatoma cells, in vitro and in vivo, is investigated. EESP (50-500 mu g/ml) exhibits significant cytotoxicity on HepG2 cells. This antiproliferative action is partly attributable to cell cycle arrest in the G(0)/G(1) phase and apoptosis induction. EESP induces apoptosis through an increase in the Bax/Bcl-2 ratio, the down-regulation of mitochondrial membrane potential and the up-regulation of caspase-3. Excessive ROS generated by EESP contributes to apoptosis of HepG2 cells. According to the HPLC-DAD and HPLC-MS/MS analysis, nobiletin, tangeretin and another twelve compounds were present in EESP, which may account for its antiproliferative effect. When HepG2 cells are grown as xenografts in nude mice, the oral administration of EESP induces a significant decrease in tumor growth. Collectively, these results suggest that EESP could be a potential useful source for the future application of EESP in liver cancer therapy. (C) 2017 Elsevier Ltd. All rights reserved.
Relation:	Journal of Functional Foods, v.33, pp.363-375
Appears in Collections:	[Dept. of Food Science & Technology] Periodical Articles [Dept. of Health and Nutrition (including master's program)] Periodical Articles [Dept. of Recreation and Health-Care Management] Periodical Articles

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