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    標題: Biotransformation of Ergostane Triterpenoid Antcin K from Antrodia cinnamomea by Soil-Isolated Psychrobacillus sp AK 1817
    作者: Chiang, Chien-Min
    Wang, Tzi-Yuan
    Ke, An-Ni
    Chang, Te-Sheng
    Wu, Jiumn-Yih
    貢獻者: Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Acad Sinica, Biodivers Res Ctr
    Natl Univ Tainan, Dept Biol Sci & Technol
    Natl Quemoy Univ, Dept Food Sci
    關鍵字: Antrodia cinnamomea
    biotransformation
    antcin K
    triterpenoid
    Psychrobacillus
    日期: 2017-10
    上傳時間: 2018-11-30 15:50:12 (UTC+8)
    出版者: Mdpi Ag
    摘要: Antcin K is one of the major ergostane triterpenoids from the fruiting bodies of Antrodia cinnamomea, a parasitic fungus that grows only on the inner heartwood wall of the aromatic tree Cinnamomum kanehirai Hay (Lauraceae). To search for strains that have the ability to biotransform antcin K, a total of 4311 strains of soil bacteria were isolated, and their abilities to catalyze antcin K were determined by ultra-performance liquid chromatography analysis. One positive strain, AK 1817, was selected for functional studies. The strain was identified as Psychrobacillus sp., based on the DNA sequences of the 16S rRNA gene. The biotransformation metabolites were purified with the preparative high-performance liquid chromatography method and identified as antcamphin E and antcamphin F, respectively, based on the mass and nuclear magnetic resonance spectral data. The present study is the first to report the biotransformation of triterpenoids from A. cinnamomea (Antrodia cinnamomea).
    關聯: Catalysts, v.7, n.10, 文獻號碼:299
    Appears in Collections:[生物科技系(所)] 期刊論文

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