English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18033/20233 (89%)
造訪人次 : 23241687      線上人數 : 295
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/31328


    標題: Immunomodulatory effects of Chlorella aqueous extract on IL-12 and IL-10 production in macrophage and LPS-induced sepsis in mice
    作者: 李芝綺
    鄭涵勻
    貢獻者: 嘉南藥理大學藥學系
    施美份
    日期: 2016-05-10
    上傳時間: 2018-05-21 10:34:45 (UTC+8)
    摘要: Objectives: Immunity balance is regulated between immune activation and immune inhibition. Interleukin-12 (IL-12) has an essential role in the interaction between the innate and adaptive arms of immunity by regulating inflammatory responses, innate resistance to infection, and adaptive immunity. In addition, IL-12 is critical to an efficient host defense in polymicrobial sepsis. On the other hand, in response to inflammatory activation during LPS challenge, host cells also produce anti-inflammatory cytokines like interleukin-10 (IL-10). Imbalance between pro-inflammatory and anti-inflammatory response can result in circulatory failure, organ dysfunction, the immunosuppressive state and even death. Chlorella aqueous extract has been demonstrated to augment resistance against challenge with tumor cells in mice, which may be mediated through the participation of T cells and macrophages. However, immunomodulatory effects of Chlorella aqueous extract (doses between 2 and 0.25 mg/ml) were unknown. Methods: Effects of Chlorella aqueous extract on NO, TNF-α, and IL-10 productions and IL-12 gene expression in RAW264.7 macrophages were compared to LPS. Sepsis was induced by LPS (i.p.). Results and Conclusion: Although the level of NO production in Chlorella-treated cells was less than that obtained in LPS-stimulated cells, the production of TNF-α was comparable to LPS-stimulated group. The induction of IL-10 by Chlorella aqueous extract was greater than that seen in LPS-stimulated group. In addition, Chlorella aqueous extract (2 and 1 mg/ml) also enhanced IL-12p40 gene expression. LPS-induced significant mortality in mice, Chlorella aqueous extract (20 mg/kg) application improved the survival rate in the LPS-induced sepsis mice. Taken together, protective effects of Chlorella aqueous extract from LPS-induced sepsis may be mediated through counterbalance of anti-inflammatory cytokine IL-10 and proinflammatory cytokine IL-12 and TNF-α production.
    關聯: 2016 第十屆嘉南藥理大學藥理學院師生研究成果發表會,起迄日:2016/05/10-2016/05/13,地點:嘉南藥理大學國際會議中心一樓
    顯示於類別:[藥學系(所)] 會議論文
    [藥理學院] 2016 第十屆嘉南藥理大學藥理學院師生研究成果發表會

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    B03.pdf470KbAdobe PDF233檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋