The role of doxycycline in the therapy of multidrug-resistant E. coli - an in vitro study

doi:10.1038/srep31964

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Title:	The role of doxycycline in the therapy of multidrug-resistant E. coli - an in vitro study
Authors:	Lai, Chih-Cheng Chen, Chi-Chung Huang, Hui-Ling Chuang, Yin-Ching Tang, Hung-Jen
Contributors:	Chi Mei Med Ctr, Dept Intens Care Med Chi Mei Med Ctr, Dept Med Res Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chi Mei Med Ctr, Dept Internal Med Chi Mei Med Ctr, Dept Med
Keywords:	carbapenemase-producing enterobacteriaceae glycylcycline antimicrobial agent field gel-electrophoresis gram-negative bacteria klebsiella-pneumoniae escherichia-coli pseudomonas-aeruginosa combination therapy beta-lactamases infections
Date:	2016-08
Issue Date:	2018-01-18 11:41:00 (UTC+8)
Publisher:	Nature Publishing Group
Abstract:	This study assessed the in vitro antibacterial activity of combinations of amikacin and doxycycline or tigecycline against multidrug-resistant E. coli isolates. Twenty-four different pulsotypes, including 10 extended-spectrum beta-lactamase (ESBL)-, 10 carbapenem-resistant, 2 New Delhi Metallo-beta-lactamase (NDM)- and 2 Klebsiella pneumoniae carbapenemase (KPC)-E. coli isolates were collected. All 24 isolates were susceptible to amikacin and tigecycline. Only 30% of ESBL and 50% of carbapenem-resistant E. coli were susceptible to doxycycline. Both of the NDM-E. coli had a MIC of 64 mu g/ml. The checkerboard method showed that for the ESBL-and carbapenem-resistant E. coli, the synergistic effects of amikacin/doxycycline were 80% and 90%, respectively. For the two KPC- and two NDM-E. coli, the FIC index of amikacin/doxycycline were 0.5/0.375 and 0.5/0.281, respectively. For the ESBL- and carbapenem-resistant E. coli isolates, the combinations of amikacin and doxycycline exhibited synergistic activities against 80%, and 80% and 10% vs 60%, and 80% and 10% of the isolates at concentrations of 1x, 1/2x and 1/4xMIC, respectively. The synergistic effect seems to be similar for doxycycline and tigecycline based combinations with amikacin. In conclusion, the antibacterial activity of doxycycline can be enhanced by the addition of amikacin and is observed against most multidrug-resistant E. coli isolates.
Relation:	Scientific Reports, v.6, 31964
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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