Chemotherapeutic effects of luteolin on radio-sensitivity enhancement and interleukin-6/signal transducer and activator of transcription 3 signaling repression of oral cancer stem cells

doi:10.1016/j.jfma.2016.08.009

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標題:	Chemotherapeutic effects of luteolin on radio-sensitivity enhancement and interleukin-6/signal transducer and activator of transcription 3 signaling repression of oral cancer stem cells
作者:	Tu, Dom-Gene Lin, Wei-Ting Yu, Cheng-Chia Lee, Shiuan-Shinn Peng, Chih-Yu Lin, Taichen Yu, Chuan-Hang
貢獻者:	Ditmanson Med Fdn, Chia Yi Christian Hosp, Dept Nucl Med Chia Nan Univ Pharm & Sci, Dept Food Sci & Technol Chang Jung Christian Univ, Coll Hlth Sci Ditmanson Med Fdn, Chia Yi Christian Hosp, Dept Oral & Maxillofacial Surg Chung Shan Med Univ, Sch Dent Chung Shan Med Univ Hosp, Dept Dent Chung Shan Med Univ, Inst Oral Sci Chung Shan Med Univ, Sch Publ Hlth
關鍵字:	cancer stem cells luteolin oral carcinomas radio-sensitivity
日期:	2016-12
上傳時間:	2018-01-18 11:38:48 (UTC+8)
出版者:	Elsevier Taiwan
摘要:	Background/Purpose: Previously, we successfully identified oral cancer stem cells (OCSC) displaying enhanced stemness and tumorigenic potentials. In the study, we investigated the chemotherapeutic effect of the flavonoid luteolin, commonly found in fruits and vegetables, on targeting OCSC. Methods: Oralspheres was applied to isolate OCSC. aldehyde dehydrogenase 1 activity and CD44 positivity of OCSC with luteolin treatment were assessed by flow cytometry analysis. Radio-sensitivity of OCSC treated with luteolin was examined. Invasion and colony-forming assays were performed to assess oncogenicity in OCSC. The expression of interleukin-6 (IL-6)/ signal transducer and activator of transcription 3 (STAT3) was examined by enzyme-linked immunosorbent assay and western blot analysis. Results: We showed that luteolin effectively inhibited the proliferation rate, self-renewal, aldehyde dehydrogenase 1 activity, and CD44 positivity of OCSC but did not cause significant cytotoxicity of normal epithelial cells. Moreover, luteolin restored radio-sensitivity in OCSC. Combined treatment with luteolin and radiation displayed synergistic effect on invasiveness and clonogenicity of OCSC. Mechanistically, treatment of luteolin resulted in inactivation of IL-6/STAT3 signaling. Conclusion: These results suggest that combined treatment of luteolin and radiation therapy can attenuate tumorigenicity of OCSC through IL-6/STAT3 signaling inactivation. Copyright (C) 2016, Formosan Medical Association. Published by Elsevier Taiwan LLC. This is an open access article under the CC BY-NC-ND license.
關聯:	Journal of the Formosan Medical Association, v.115 n.12, pp.1032-1038
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