English  |  正體中文  |  简体中文  |  Items with full text/Total items : 16812/19099 (88%)
Visitors : 6362828      Online Users : 337
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30947


    標題: Improving Free Radical Scavenging Activity of Soy Isoflavone Glycosides Daidzin and Genistin by 3 '-Hydroxylation Using Recombinant Escherichia coli
    作者: Chiang, Chien-Min
    Wang, Dong-Sheng
    Chang, Te-Sheng
    貢獻者: Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Natl Univ Tainan, Dept Biol Sci & Technol
    關鍵字: antioxidant
    biotransformation
    daidzin
    genistin
    hydroxylation
    日期: 2016-12
    上傳時間: 2018-01-18 11:38:29 (UTC+8)
    出版者: Mdpi Ag
    摘要: The present study describes the biotransformation of a commercially available crude extract of soy isoflavones, which contained significant amounts of the soy isoflavone glycosides daidzin and genistin, by recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium. Two major products were isolated from the biotransformation and identified as 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively, based on their mass and nuclear magnetic resonance spectral data. The two 3'-hydroxyisoflavone glycosides showed potent 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity with IC50 values of 7.4 and 9.8 mu M for 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively. The free radical scavenging activities of the two 3'-hydroxyisoflavone glycosides were, respectively, 120 and 72 times higher than the activity of their precursors, daidzin and genistin, and were also stronger than the activity of ascorbic acid, which showed an IC50 value of 15.1 mu M. This is the first report of the bio-production and potential antioxidant applications of both 3'-hydroxydaidzin and 3'-hydroxygenistin.
    關聯: Molecules, v.21 n.12, 1723
    Appears in Collections:[生物科技系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat
    30947.pdf1664KbAdobe PDF33View/Open
    index.html0KbHTML140View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback