IL-8 promotes HNSCC progression on CXCR1/2-meidated NOD1/RIP2 signaling pathway

doi:10.18632/oncotarget.11445

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/30918

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30918

Title:	IL-8 promotes HNSCC progression on CXCR1/2-meidated NOD1/RIP2 signaling pathway
Authors:	Chan, Leong-Perng Wang, Ling-Feng Chiang, Feng-Yu Lee, Ka-Wo Kuo, Po-Lin Liang, Chia-Hua
Contributors:	Kaohsiung Med Univ, Grad Inst Clin Med Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Otolaryngol Head & Neck Surg Natl Sun Yat Sen Univ, Inst Med Sci & Technol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Chia Nan Univ Pharm & Sci, Inst Cosmet Sci
Keywords:	HNSCC IL-8 NOD1 cancer progression inflammasomes
Date:	2016-09
Issue Date:	2018-01-18 11:37:55 (UTC+8)
Publisher:	Impact Journals Llc
Abstract:	NOD1 (nucleotide-binding oligomerization domain 1) is overexpressed in head and neck squamous cell carcinoma (HNSCC) cells, as is IL-8 in cancer cells. However, the mechanism of the IL-8-mediated overexpression of NOD in HNSCC not been identified. This study determines whether IL-8 promotes tumor progression via the NOD signaling pathway in HNSCC. Higher IL-8, NOD1 and receptor-interacting protein kinase (RIP2) expressions were observed in HNSCC tissue than in non-cancerous matched tissue (NCMT), whereas NOD2 was weakly expressed. Furthermore, IL-8 stimulated the proliferation of HNSCC cells (SCC4, SCC9 and SCC25) but not dysplastic oral mucosa DOK cells. Exposure to IL-8 increased the clonogenicity of HNSCC cells. IL-8 siRNA inhibited cell proliferation and cell colony formation, suggesting that IL-8 is involved in HNSCC cancer progression. The expressions of CXCR1 and CXCR2 were higher in HNSCC tissue than in NCMT. HNSCC cells that were exposed to IL-8 exhibited higher expression of CXCR1/2 than did controls. The blocking of IL-8 by siRNA reduced CXCR1/2 expression in HNSCC cells, suggesting that the cancer progression of HNSCC cells that is induced by IL-8 depends on CXCR1/2. Additionally, IL-8 is associated with increased NOD1 and RIP2 expression and reduced NOD2 expression in three types of HNSCC cells. The blocking of IL-8 by siRNA reduces IL-8, NOD1 and RIP2 expressions in HNSCC cells, but not the level of NOD2. These results suggest that IL-8 has an important role in HNSCC progression via a CXCR1/2-meidated NOD1/RIP2 signaling pathway.
Relation:	Oncotarget, v.7 n.38, pp.61820-61831
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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