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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30907


    標題: Reactive Oxygen Species Mediate Terbufos-Induced Apoptosis in Mouse Testicular Cell Lines via the Modulation of Cell Cycle and Pro-Apoptotic Proteins
    作者: Hung, Jui-Hsiang
    Chen, Chia-Yun
    Omar, Hany A.
    Huang, Kuo-Yuan
    Tsao, Che-Chia
    Chiu, Chien-Chih
    Chen, Yi-Ling
    Chen, Po-Han
    Teng, Yen-Ni
    貢獻者: Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Chia Nan Univ Pharm & Sci, Drug Discovery & Dev Ctr
    Beni Suef Univ, Fac Pharm, Dept Pharmacol, Bani Suwayf 62514, Egypt
    Univ Sharjah, Sharjah Inst Med Res, Dept Pharmacol, Coll Pharm
    Natl Cheng Kung Univ, Dept Orthoped, Coll Med
    Natl Univ Tainan, Dept Biol Sci & Technol
    Kaohsiung Med Univ, Dept Biotechnol
    Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
    Far East Univ, Dept Cosmet Applicat & Management
    關鍵字: terbufos
    caspase-3
    reactive oxygen species
    rats
    日期: 2016-12
    上傳時間: 2018-01-18 11:37:41 (UTC+8)
    出版者: Wiley-Blackwell
    摘要: Terbufos (S-t-butylthiomethyl-O, O-diethyl phosphorodithioate) is a highly toxic organophosphate which is extensively used as an insecticide and nematicide. Chronic exposure to terbufos causes neuronal injury and predisposes to neurodegenerative diseases. Accumulating evidence has shown that the exposure to terbufos, as an occupational risk factor, may also cause reproductive disorders. However, the exact mechanisms of reproductive toxicity remain unclear. The present study aimed to investigate the toxic effect of terbufos on testicular cells and to explore the mechanism of toxicity on a cellular level. The cytotoxic effects of terbufos on mouse immortalized spermatogonia (GC-1), spermatocytes (GC-2), Leydig (TM3), and Sertoli (TM4) cell lines were assessed by MTT assays, caspase activation, flow cytometry, TUNEL assay, Western blot, and cell cycle analysis. The exposure to different concentrations of terbufos ranging from 50 to 800 mu M for 6 h caused significant death in all the used testicular cell lines. Terbufos increased reactive oxygen species (ROS) production, reduced mitochondrial membrane potential, and initiated apoptosis, which was confirmed by a dose-dependent increase in the number of TUNEL-positive apoptotic cells. Blocking ROS production by N-acetyl cysteine (NAC) protected GC-1 cells from terbufos-induced cell death. The results demonstrated that terbufos induces ROS, apoptosis, and DNA damage in testicular cell lines and it should be considered potentially hazardous to testis. Together, this study provided potential molecular mechanisms of terbufos-induced toxicity in testicular cells and suggests a possible protective measure. (C) 2015 Wiley Periodicals, Inc.
    關聯: Environmental Toxicology, v.31 n.12, pp.1888-1898
    Appears in Collections:[老人服務事業管理系] 期刊論文
    [生物科技系(所)] 期刊論文

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