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    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/30906


    標題: Development of Genetic Dereplication Strains in Aspergillus nidulans Results in the Discovery of Aspercryptin
    作者: Chiang, Yi-Ming
    Ahuja, Manmeet
    Oakley, C. Elizabeth
    Entwistle, Ruth
    Asokan, Anabanadam
    Zutz, Christoph
    Wang, Clay C. C.
    Oakley, Berl R.
    貢獻者: Univ So Calif, Sch Pharm, Dept Pharmacol & Pharmaceut Sci
    Chia Nan Univ Pharm & Sci, Dept Pharm
    Univ Kansas, Dept Mol Biosci
    Univ Vet Med Vienna, Inst Milk Hyg
    Univ So Calif, Dept Chem
    Univ Kansas, Del Shankel Struct Biol Ctr
    Reliance Ind Ltd, Reliance Technol Grp, Ind Biotechnol Div
    關鍵字: aspergillus
    biosynthesis
    genetic dereplication
    natural products
    NRPS
    flavus
    日期: 2016-01
    上傳時間: 2018-01-18 11:37:40 (UTC+8)
    出版者: Wiley-V C H Verlag Gmbh
    摘要: To reduce the secondary metabolite background in Aspergillus nidulans and minimize the rediscovery of compounds and pathway intermediates, we created a "genetic dereplication" strain in which we deleted eight of the most highly expressed secondary metabolite gene clusters (more than 244,000 base pairs deleted in total). This strain allowed us to discover a novel compound that we designate aspercryptin and to propose a biosynthetic pathway for the compound. Interestingly, aspercryptin is formed from compounds produced by two separate gene clusters, one of which makes the well-known product cichorine. This raises the exciting possibility that fungi use differential regulation of expression of secondary metabolite gene clusters to increase the diversity of metabolites they produce.
    關聯: Angewandte Chemie-International Edition, v.55 n.5, pp.1662-1665
    Appears in Collections:[藥學系(所)] 期刊論文

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