|摘要: ||牛樟芝（Antrodia cinnamomea, AC）是一種台灣特有的藥用真菌，AC的生理活性包含護肝、抗發炎、抗C型肝炎病毒及抗癌等；因此，AC已成為新藥開發及保健食品研發的重要原料。中草藥及藥物奈米化已被證實可增加溶解度、生物利用率、減少藥物毒性、強化藥理活性等功能；但目前很少有奈米化AC之活性物質含量及生理活性的文獻報導。本實驗嘗試比較一般研磨牛樟芝子實體（Ground Antrodia cinnamomea fruiting body, GACFB）及奈米研磨牛樟芝子實體（Nanonized Antrodia cinnamomea fruiting body, NACFB）其三萜類、多醣體含量及以LPS誘導發炎的RAW264.7細胞之抗發炎活性之差異。結果顯示NACFB 在UPLC-MS/MS分析之三萜類含量antcin K、antcin H、 antcin B、antcin C、dehydroeburicoic acid等5種三萜類的含量分別比GACFB高出 67%、 66%、 54%、 53% 及 51%。NACFB多醣體含量較GACFB高18.51%，並具有顯著差異。在RAW264.7細胞抗發炎活性中NACFB在6.25 μg/mL下對MAPKs訊號之磷酸化JNK、ERK1/2、p38表現量抑制效果顯著高於GACFB 13.8%、24.8%及34.8%。在PGE2表現量與細胞核之轉錄因子p65表現量顯著高於GACFB 10.8%、 28.3%。在IL-6、IL-10抑制效果顯著高於GACFB 45.84 %、81.27%，結果顯示在相同的濃度下NACFB比GACFB更能有效的抑制由LPS誘導RAW264.7細胞的發炎反應。|
Antrodia cinnamomea (AC), a Taiwan-specific Medicinal mushroom, has been reported to have numerous biological activities including hepatoprotection, anti-inflammation, antihepatitis C virus activity, and anticancer activity. It has been used in the formulation of pharmaceutical and functional foods. Nanotechnology has large number of advantages for herb or drugs, including enhancement of solubility and bioavailability, protection from toxicity, enhancement of pharmacological activity, enhancement of stability, improving tissue macrophages distribution, sustained delivery, protection from physical and chemical degradation. However, very few researches have been done to investigate the effects of nanoization on the chemical composition and biological functions of AC. The amount of crude polysaccharides, triterpenoids and anti-inflammatory activity of Ground Antrodia cinnamomea fruiting body (GACFB) and Nanonged Antrodia cinnamomea fruiting body (NACFB) in RAW264.7 macrophages treated with LPS were compared in this study. The results from UPLC-MS/MS analyses showed the amount of triterpenoids (antcin K, antcin H, antcin B, antcin C, dehydroeburicoic acid) in NACFB were increased 67%, 66%, 54%, 53% and 51% respectively when comparied with GACFB. The amount of polysaccharide increased 18.51% after nanoization. The inhibitory effects of NACFB on phosphor-JNK, ERK1/2, p38, p65, PGE2, IL-6 and IL-10 in RAW264.7 cells was significantly higher than that of GACFB (13.25, 24.8, 34.8, 28.3, 10.8, 45.84 and 81.27%) at the concentration of 6.25 μg/mL. The results from this study indicate the anti-inflammatory activity of Antrodia cinnamomea fruiting body was increased significantly after nanoization.